1 Grupo de Biotecnologia Molecular, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
2 Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, Brazil.
3 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.

The characterization of the endoglucanase Cel12A from Gloeophyllum trabeum reveals an enzyme highly active on ß-glucan.

PLoS One. 2014;9(9):e108393

Authors: Miotto LS, de Rezende CA, Bernardes A, Serpa VI, Tsang A, Polikarpov I

Abstract

The basidiomycete fungus Gloeophyllum trabeum causes a typical brown rot and is known to use reactive oxygen species in the degradation of cellulose. The extracellular Cel12A is one of the few endo-1,4-ß-glucanase produced by G. trabeum. Here we cloned cel12A and heterologously expressed it in Aspergillus niger. The identity of the resulting recombinant protein was confirmed by mass spectrometry. We used the purified GtCel12A to determine its substrate specificity and basic biochemical properties. The G. trabeum Cel12A showed highest activity on ß-glucan, followed by lichenan, carboxymethylcellulose, phosphoric acid swollen cellulose, microcrystalline cellulose, and filter paper. The optimal pH and temperature for enzymatic activity were, respectively, 4.5 and 50 °C on ß-glucan. Under these conditions specific activity was 239.2 ± 9.1 U mg(-1) and the half-life of the enzyme was 84.6 ± 3.5 hours. Thermofluor studies revealed that the enzyme was most thermal stable at pH 3. Using ß-glucan as a substrate, the Km was 3.2 ± 0.5 mg mL(-1) and the Vmax was 0.41 ± 0.02 µmol min(-1). Analysis of the effects of GtCel12A on oat spelt and filter paper by scanning electron microscopy revealed the morphological changes taking place during the process.

PMID: 25251390 [PubMed - indexed for MEDLINE]