1 Japanese Geotechnical Association for Housing Disaster Prevention, 1622, Oshikiri, Shimizu-ku, Shizuoka 424-0008, Japan.
2 Department of Geotechnical Engineering, Institute of Civil Engineering, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
3 Department of Building, Civil and Environmental Engineering, Concordia University, 1455 de Maisonneuve Blvd. W., Montreal, QC H3G 1M8, Canada.
4 Fudo Tetra Co., 7-2, Koami-Cho, Nihonbashi, Chuo-ku, Tokyo 103-0016, Japan.

As a response to aging of cultured urease-producing microorganisms, the blending method was examined to obtain the required carbonate production amount using the apparent viability (Rcv) based on previous research. As a result, a significantly higher carbonate content than the amount of CaCl₂ 2H₂O used was produced. Since this trend has been obtained in previous studies, it was judged that carbonate hydrate was formed. As a next step, a penetration test of soil-biocement-liquid (BCS) was conducted to investigate the properties and behavior of the BCS system, taking into account the microscopic properties of the BCS response. The depth distribution of carbonate content (C) was measured by the acid dissolution method of soil sampled from the specimen. It was assumed that the C-profile was formed by adsorption based on the diffuse double layer of microorganisms. It was shown that the amount of precursor-carbonate (precursor CPR), the optical density (OD) of viable bacteria, and the physical amount of soil adsorbed at that position can be estimated from C obtained at the various depths. In addition, the previously obtained formulas among CPR, viable OD, and Rcv shown are briefly explained in this paper.