1 Laboratoire de sciences judiciaires et de médecine légale, Department of Toxicology, 1701 rue Parthenais, Montréal, Québec, Canada H2K 3S7.
2 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec, Canada H4B 1R6.
3 Department of Mathematics, Université de Sherbrooke, 2500 boulevard de l'Université, Sherbrooke, Québec, CanadaJ1K 2R1.
4 Laboratoire de sciences judiciaires et de médecine légale, Department of Toxicology, 1701 rue Parthenais, Montréal, Québec, CanadaH2K 3S7.
5 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec, CanadaH4B 1R6.

In the first part of this paper (I-Description and application), an automated, stepwise and analyst-independent process for the selection and validation of calibration models was put forward and applied to two model analytes. This second part presents the mathematical reasoning and experimental work underlying the selection of the different components of this procedure. Different replicate analysis designs (intra/inter-day and intra/inter-extraction) were tested and their impact on test results...