Author(s): Plissonneau C; Tam BT; Garneau PY; Morais JA; Piekny A; Santosa S;

Transwell co-culture systems are models for examining cellular crosstalk, but the impact of porous membrane material on myogenic outcomes remains uncharacterized. We investigated the influence of transwell membrane material on myoblast differentiation in co-culture with human primary adipocytes and in varying concentrations of insulin-transferrin-selenium ...

Article GUID: 42260998

Author(s): Delaney KZ; Nadeem MI; Plissonneau C; Khor N; Delaney J; Morais JA; Tsoukas MA; Pescarus R; Garneau PY; Santosa S;

Adipose tissue (AT) immune cells are implicated in the pathology of type 2 diabetes (T2D), and their content likely differs between abdominal subcutaneous adipose tissue (abSAT), femoral SAT (fmSAT), and visceral adipose tissue (VAT). Therefore, the objective of the current study was to develop a ...

Article GUID: 42255513

Author(s): Wannaiampikul S; Lee B; Chen J; Prentice KJ; Ayansola R; Xu A; Santosa S; Pantopoulos K; Sweeney G;

Background: Metabolic Syndrome (MetS) presents a global health challenge, characterized by obesity, hypertension, dyslipidemia, and insulin resistance. Despite recognition of the gut microbiome's role in metabolic health, there remains scope for defining association of unique microbes with cl ...

Article GUID: 41794383

Author(s): Murphy J; Morais JA; Tsoukas MA; Cooke AB; Daskalopoulou SS; Santosa S;

Introduction: Adipose tissue inflammation, driven in part by immune cells, may contribute to the elevated type 2 diabetes risk in adults with childhood-onset obesity (CO) compared to those with adult-onset obesity (AO). Weight loss can modify adipose tissue immune cell composition, but whether these changes differ by obesity onset remains unknown. Method ...

Article GUID: 40831565

Author(s): Turner L; Wanasinghe AI; Brunori P; Santosa S;

In individuals with obesity, the onset of chronic comorbidities coincides with the excessive accumulation of adipose tissue in various tissue beds. As obesity progresses, adipose tissue becomes increasingly dysfunctional causing chronic low-grade inflammation. Indeed, adipose tissue inflammation, which partially stems from macrophage infiltration and expr ...

Article GUID: 40533358

Author(s): Tam BT; Wan K; Santosa S; Cai Z;

With over 420 million children (aged 0-19 years) worldwide living with overweight or obesity, the "obesity epidemic" or "globesity" is a defining public health challenge of this generation. While significant efforts have been made to address obesity in adults, less attention has been given to the critical issue of childhood overweight and ...

Article GUID: 39991475

Author(s): Feola S; Al-Nabelsi L; Tam BT; Near J; Morais JA; Santosa S;

Objective: Compared to adulthood-onset obesity (AO), those with childhood-onset obesity (CO) are at greater risk of metabolic disease. However, the differences between these two obesity phenotypes are not clear. The aim of this study is to investigate how the age of obesity onset (CO vs. AO) affects the use of intramyocellular (IMCL) and extramyocellular ...

Article GUID: 39875595

Author(s): Costa DN; Santosa S; Jensen MD;

Adult males and females have markedly different body composition, energy expenditure, and have different degrees of risk for metabolic diseases. A major aspect of metabolic regulation involves the appropriate storage and disposal of glucose and fatty acids. The use of sophisticated calorimetry, tracer, and imaging techniques have provided insight into the ...

Article GUID: 39869194

Author(s): Plissonneau C; Santosa S;

The excessive accumulation of adipose tissue in obesity appears to result in adipose tissue dysfunction perpetuating the onset of obesity-related diseases, including type 2 diabetes (T2DM). In humans, adipose tissue is stored in several depots including subcutaneous and visceral. These depots contribute to the pathology of obesity differently owing to dif ...

Article GUID: 39553621

Author(s): Murphy J; Dera A; Morais JA; Tsoukas MA; Khor N; Sazonova T; Almeida LG; Cooke AB; Daskalopoulou SS; Tam BT; Santosa S;

Objective: We aimed to examine the effect of age of obesity onset, sex, and their interaction on abdominal and femoral subcutaneous adipose tissue (SAT) morphology (degree of adipocyte hyperplasia or hypertrophy). Methods: In this cross-sectional study, we isolated adipocytes via collagenase dig ...

Article GUID: 39045668

Author(s): Sedemedes K; Knäuper B; Sadikaj G; Yuan TY; Wrosch C; Santosa S; Alberga AS; Kakinami L;

The "Compensatory Health Beliefs" scale assesses the degree to which one believes that unhealthy behaviours can be compensated through healthier ones. However, no validated scale to assess compensatory weight-related behaviors exists. The study's objective was to develop (Study 1) and validate (Study 2) a questionnaire measuring compensatory health mo ...

Article GUID: 37804879