Author(s): Delaney KZ; Nadeem MI; Plissonneau C; Khor N; Delaney J; Morais JA; Tsoukas MA; Pescarus R; Garneau PY; Santosa S;

Adipose tissue (AT) immune cells are implicated in the pathology of type 2 diabetes (T2D), and their content likely differs between abdominal subcutaneous adipose tissue (abSAT), femoral SAT (fmSAT), and visceral adipose tissue (VAT). Therefore, the objective of the current study was to develop a ...

Article GUID: 42255513

Author(s): Hajiaghayi M; Gholizadeh F; Rahbari N; Emamnia N; Shih SCC; Darlington PJ;

Introduction: Cancers often drive T cells toward an exhausted state characterized by impaired cytotoxicity and upregulation of inhibitory receptors (PD-1, TIM-3, CD38) and transcriptional regulators (TOX, NFATc1). Repeated stimulation in vitro is used to model this process, reflecting chronic antigen exposure in the tumor microenvironment. Stress-derived ...

Article GUID: 41906691

Author(s): Baez A; Hazel K; Guertin Z; Fong E; Manus MM; Kaloyannis A; Helfield B;

Adoptive cell transfer (ACT)-based immunotherapy has emerged as a transformative approach for treating cancer, offering durable responses through the ex vivo expansion and reinfusion of antigen-specific immune cells. Despite remarkable clinical successes, most notably with chimeric antigen receptor (CAR)-T cell therapy, ACT remains limited by severe toxic ...

Article GUID: 41748028

Author(s): Murphy J; Morais JA; Tsoukas MA; Cooke AB; Daskalopoulou SS; Santosa S;

Introduction: Adipose tissue inflammation, driven in part by immune cells, may contribute to the elevated type 2 diabetes risk in adults with childhood-onset obesity (CO) compared to those with adult-onset obesity (AO). Weight loss can modify adipose tissue immune cell composition, but whether these changes differ by obesity onset remains unknown. Method ...

Article GUID: 40831565

Author(s): Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS;

GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei C ...

Article GUID: 40622460

Author(s): Carvajal Gonczi CM; Hajiaghayi M; Gholizadeh F; Xavier Soares MA; Touma F; Lopez Naranjo C; Rios AJ; Pozzebon C; Daigneault T; Burchell-Reyes K; Darlington PJ;

Background: T helper 17 (Th17) cells produce IL-17A cytokine and can exacerbate autoimmune diseases and asthma. The ß2 adrenergic receptor is a g protein-coupled receptor that induces cAMP second messenger pathways. We tested the hypothesis that terbutaline, a ß2-adrenergic receptor-specific agon ...

Article GUID: 37438188

Author(s): Reijngoud J; Arentshorst M; Ruijmbeek C; Reid I; Alazi ED; Punt PJ; Tsang A; Ram AFJ;

Objective: With the aim to decipher the mechanisms involved in the transcriptional regulation of feruloyl esterase encoded by faeB, a genetic screen was performed to isolate A. niger mutants displaying inducer-independent expression from the faeB promoter. Result: PfaeB-amdS and PfaeB-lux dual reporter strains were constructed and used to isolate trans-a ...

Article GUID: 33738610

Author(s): Wang C; Murphy J; Delaney KZ; Khor N; Morais JA; Tsoukas MA; Lowry DE; Mutch DM; Santosa S;

Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study ...

Article GUID: 33595419

Author(s): Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ

Glia. 2019 04;67(4):582-593 Authors: Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ

Article GUID: 30444064