Author(s): Narcross L; Pyne ME; Kevvai K; Siu KH; Dueber JE; Martin VJJ;

The benzylisoquinoline alkaloid (BIA) family of tetrahydroisoquinolines (THIQs) comprises >2,500 members, including the pharmaceuticals morphine, codeine, and papaverine, as well as the antibiotics sanguinarine and chelerythrine. Agricultural cultivation can supply the demand for the BIAs that accumulate in plants, but broader access to the BIA family wou ...

Article GUID: 41779670

Author(s): Dykstra CB; Pyne ME; Martin VJJ;

A fundamental challenge of metabolic engineering involves assembling and screening vast combinations of orthologous enzymes across a multistep biochemical pathway. Current pathway assembly workflows involve combining genetic parts ex vivo and assembling one pathway configuration per tube or well. Here, we present CRAPS, Chromosomal-Repair-Assisted Pathway ...

Article GUID: 37584634

Author(s): Pyne ME; Gold ND; Martin VJJ;

Sacred lotus (Nelumbo nucifera) has been utilized as a food, medicine, and spiritual symbol for nearly 3000 years. The medicinal properties of lotus are largely attributed to its unique profile of benzylisoquinoline alkaloids (BIAs), which includes potential anti-cancer, anti-malarial and anti-arrhythmic compounds. BIA biosynthesis in sacred lotus differs ...

Article GUID: 37004909

Author(s): Pyne ME, Narcross L, Melgar M, Kevvai K, Mookerjee S, Leite GB, Martin VJJ

Appl Environ Microbiol. 2018 Sep 01;84(17): Authors: Pyne ME, Narcross L, Melgar M, Kevvai K, Mookerjee S, Leite GB, Martin VJJ

Article GUID: 29934332

Author(s): Bourgeois L, Pyne ME, Martin VJJ

ACS Synth Biol. 2018 Nov 16;7(11):2675-2685 Authors: Bourgeois L, Pyne ME, Martin VJJ

Article GUID: 30372609

Author(s): Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Methods Enzymol. 2016;575:195-224 Authors: Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Article GUID: 27417930