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The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit

Author(s): Bean BDM; Whiteway M; Martin VJJ;

The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-bas ...

Article GUID: 35708612


Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Author(s): Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; ...

The yeast Saccharomyces cerevisiae is powerful for studying human G protein-coupled receptors as they can be coupled to its mating pathway. However, some receptors, including the mu opioid receptor ...

Article GUID: 35610225


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