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Thermotolerance in S. cerevisiae as a model to study extracellular vesicle biology

Author(s): Logan CJ; Staton CC; Oliver JT; Bouffard J; Kazmirchuk TDD; Magi M; Brett CL;

The budding yeast Saccharomyces cerevisiae is a proven model organism for elucidating conserved eukaryotic biology, but to date its extracellular vesicle (EV) biology is understudied. Here, we show yeast transmit information through the extracellular medium ...

Article GUID: 38711329


A two-tiered system for selective receptor and transporter protein degradation

Author(s): Golden CK; Kazmirchuk TDD; McNally EK; El Eissawi M; Gokbayrak ZD; Richard JD; Brett CL;

Diverse physiology relies on receptor and transporter protein down-regulation and degradation mediated by ESCRTs. Loss-of-function mutations in human ESCRT genes linked to cancers and neurological disorders are thought to block this process. However, when h ...

Article GUID: 36215320


Acetate and hypertonic stress stimulate vacuole membrane fission using distinct mechanisms

Author(s): Gokbayrak ZD; Patel D; Brett CL;

Vacuoles in plants and fungi play critical roles in cell metabolism and osmoregulation. To support these functions, vacuoles change their morphology, e.g. they fragment when these organisms are challenged with draught, high salinity or metabolic stress (e.g ...

Article GUID: 35834522


Distinct features of multivesicular body-lysosome fusion revealed by a new cell-free content-mixing assay.

Author(s): Karim MA, Samyn DR, Mattie S, Brett CL

Traffic. 2018 02;19(2):138-149 Authors: Karim MA, Samyn DR, Mattie S, Brett CL

Article GUID: 29135058


The Na+(K+)/H+ exchanger Nhx1 controls multivesicular body-vacuolar lysosome fusion.

Author(s): Karim MA, Brett CL

Mol Biol Cell. 2018 02 01;29(3):317-325 Authors: Karim MA, Brett CL

Article GUID: 29212874


Rab-Effector-Kinase Interplay Modulates Intralumenal Fragment Formation during Vacuole Fusion.

Author(s): Karim MA, McNally EK, Samyn DR, Mattie S, Brett CL

Dev Cell. 2018 10 08;47(1):80-97.e6 Authors: Karim MA, McNally EK, Samyn DR, Mattie S, Brett CL

Article GUID: 30269949


A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion.

Author(s): Karim MA, Samyn DR, Brett CL

Methods Mol Biol. 2019;1860:289-301 Authors: Karim MA, Samyn DR, Brett CL

Article GUID: 30317513


Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.

Author(s): Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Methods Mol Biol. 2019;1860:361-377 Authors: Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Article GUID: 30317518


The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.

Author(s): McNally EK, Brett CL

Nat Commun. 2018 12 18;9(1):5358 Authors: McNally EK, Brett CL

Article GUID: 30560896


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