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Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass

Author(s): Sharma G; Kaur B; Singh V; Raheja Y; Falco MD; Tsang A; Chadha BS;

Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxili ...

Article GUID: 38676717


A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulosics biomass

Author(s): Raheja Y; Singh V; Sharma G; Tsang A; Chadha BS;

The present study reports a highly thermostable ß-glucosidase (GH3) from Rasamsonia emersonii that was heterologously expressed in Pichia pastoris. Extracellular ß-glucosidase was purified to homogeneity using single step affinity chromatography with molecu ...

Article GUID: 38470501


CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii

Author(s): Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS;

Background: The filamentous fungus Rasamsonia emersonii has immense potential to produce biorefinery relevant thermostable cellulase and hemicellulase enzymes using lignocellulosic biomass. Previously in our lab, a hyper-cellulase producing strain of R. eme ...

Article GUID: 37658430


Economizing the lignocellulosic hydrolysis process using heterologously expressed auxiliary enzymes feruloyl esterase D (CE1) and β-xylosidase (GH43) derived from thermophilic fungi Scytalidium thermophilum

Author(s): Agrawal D; Tsang A; Chadha BS;

Two lignocellulolytic accessory enzymes, feruloyl esterase D (FAED_SCYTH) and ß-xylosidase (XYL43B_SCYTH) were cloned and produced in the Pichia pastoris X33 as host. The molecular weight of recombinant enzymes FAED_SCYTH and XYL43B_SCYTH were ~ 31 and 40 k ...

Article GUID: 34293687


Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.

Author(s): Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS

Appl Biochem Biotechnol. 2019 Dec 02;: Authors: Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS

Article GUID: 31792786


Malbranchea cinnamomea: A thermophilic fungal source of catalytically efficient lignocellulolytic glycosyl hydrolases and metal dependent enzymes.

Author(s): Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS

Bioresour Technol. 2016 Jan;200:55-63 Authors: Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS

Article GUID: 26476165


Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers.

Author(s): Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS

Bioresour Technol. 2016 Sep;216:958-67 Authors: Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS

Article GUID: 27341464


Expression of catalytically efficient xylanases from thermophilic fungus Malbranchea cinnamomea for synergistically enhancing hydrolysis of lignocellulosics.

Author(s): Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS

Int J Biol Macromol. 2018 Mar;108:185-192 Authors: Basotra N, Joshi S, Satyanarayana T, Pati PK, Tsang A, Chadha BS

Article GUID: 29174359


Thermostable xylanases from thermophilic fungi and bacteria: Current perspective.

Author(s): Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A

Bioresour Technol. 2019 Apr;277:195-203 Authors: Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A

Article GUID: 30679061


Characterization of a novel Lytic Polysaccharide Monooxygenase from Malbranchea cinnamomea exhibiting dual catalytic behavior

Author(s): Basotra N; Dhiman SS; Agrawal D; Sani RK; Tsang A; Chadha BS;

A novel Lytic Polysaccharide Monooxygenase (LPMO) family AA9 (PMO9A_MALCI) protein from thermophilic fungus Malbranchea cinnamomea was cloned and expressed in Pichia pastoris. The expressed protein was purified to homogeneity using ion exchange and hydropho ...

Article GUID: 31054382


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