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Erratum: Correction Notice: Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9

Author(s): Akhmetov A; Laurent JM; Gollihar J; Gardner EC; Garge RK; Ellington AD; Kachroo AH; Marcotte EM;

[This corrects the article .].

Article GUID: 38161732


Rapid, scalable, combinatorial genome engineering by marker-less enrichment and recombination of genetically engineered loci in yeast

Author(s): Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH; ...

A major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present a precise and ...

Article GUID: 37323580


Functional expression of opioid receptors and other human GPCRs in yeast engineered to produce human sterols

Author(s): Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; ...

The yeast Saccharomyces cerevisiae is powerful for studying human G protein-coupled receptors as they can be coupled to its mating pathway. However, some receptors, including the mu opioid receptor ...

Article GUID: 35610225


Discovery of new vascular disrupting agents based on evolutionarily conserved drug action, pesticide resistance mutations, and humanized yeast

Author(s): Garge RK; Cha HJ; Lee C; Gollihar JD; Kachroo AH; Wallingford JB; Marcotte EM;

Thiabendazole (TBZ) is an FDA-approved benzimidazole widely used for its antifungal and antihelminthic properties. We showed previously that TBZ is also a potent vascular disrupting agent and inhibits angiogenesis at the tissue level by dissociating vascula ...

Article GUID: 34849907


Humanization of yeast genes with multiple human orthologs reveals functional divergence between paralogs.

Author(s): Laurent JM, Garge RK, Teufel AI, Wilke CO, Kachroo AH, Marcotte EM

PLoS Biol. 2020 May 18;18(5):e3000627 Authors: Laurent JM, Garge RK, Teufel AI, Wilke CO, Kachroo AH, Marcotte EM

Article GUID: 32421706


Single-step Precision Genome Editing in Yeast Using CRISPR-Cas9.

Author(s): Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM

Bio Protoc. 2018 Mar 20;8(6): Authors: Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM

Article GUID: 29770349


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