Author(s): Hazel K; Singh D; He S; Guertin Z; Husser MC; Helfield B;

CRISPR-Cas9 ribonucleoproteins (RNPs) have been heavily considered for gene therapy due to their high on-target efficiency, rapid activity and lack of insertional mutagenesis relative to other CRISPR-Cas9 delivery formats. Genetic diseases such as hypertrophic cardiomyopathy currently lack effective treatment strategies and are prime targets for CRISPR-Ca ...

Article GUID: 39797397

Author(s): Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A;

Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered split mNeonGreen protein was used to generate a large-scale endogenous tag library in HEK293 cells. Usin ...

Article GUID: 38652106

Author(s): Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ;

Cytokinesis is required to physically separate the daughter cells at the end of mitosis. This crucial process requires the assembly and ingression of an actomyosin ring, which must occur with high fidelity to avoid aneuploidy and cell fate changes. Most of our knowledge of mammalian cytokinesis was generated using over-expressed transgenes in HeLa cells. ...

Article GUID: 36416720

Author(s): Jaunky DB; Larocque K; Husser MC; Liu JT; Forgione P; Piekny A;

We reveal the effects of a new microtubule-destabilizing compound in human cells. C75 has a core thienoisoquinoline scaffold with several functional groups amenable to modification. Previously we found that sub micromolar concentrations of C75 caused cytotoxicity. We also found that C75 inhibited microtubule polymerization and competed with colchicine for ...

Article GUID: 34880347

Author(s): Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

ACS Synth Biol. 2018 03 16;7(3):933-944 Authors: Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

Article GUID: 29516725

Author(s): Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Anal Chem. 2019 Apr 16;91(8):5159-5168 Authors: Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Article GUID: 30945840