Author(s): Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; ...

Prokaryotic transcription factors (TFs) regulate gene expression in response to small molecules, thus representing promising candidates as versatile small molecule-detecting biosensors valuable for ...

Article GUID: 38696354

Author(s): Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A;

Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered spli ...

Article GUID: 38652106

Author(s): Bagley JA; Pyne ME; Exley K; Kevvai K; Wang Q; Whiteway M; Martin VJJ;

We report draft genome sequences for 15 non-conventional Saccharomycotina yeast strains obtained from public culture repositories. Included in our collection are eight strains of Pichia with broad tolerance to dicarboxylic acids. The genome sequences of the ...

Article GUID: 37747226

Author(s): Pyne ME; Bagley JA; Narcross L; Kevvai K; Exley K; Davies M; Wang Q; Whiteway M; Martin VJJ; ...

Saccharomyces cerevisiae is a workhorse of industrial biotechnology owing to the organism's prominence in alcohol fermentation and the suite of sophisticated genetic tools available to manipula ...

Article GUID: 37652930

Author(s): Dykstra CB; Pyne ME; Martin VJJ;

A fundamental challenge of metabolic engineering involves assembling and screening vast combinations of orthologous enzymes across a multistep biochemical pathway. Current pathway assembly workflows involve combining genetic parts ex vivo and assembling one ...

Article GUID: 37584634

Author(s): Nasr MA; Martin VJJ; Kwan DH;

Reprogramming cellular behaviour is one of the hallmarks of synthetic biology. To this end, prokaryotic allosteric transcription factors (aTF) have been repurposed as versatile tools for processing small molecule signals into cellular responses. Expanding t ...

Article GUID: 37377432

Author(s): Pyne ME; Gold ND; Martin VJJ;

Sacred lotus (Nelumbo nucifera) has been utilized as a food, medicine, and spiritual symbol for nearly 3000 years. The medicinal properties of lotus are largely attributed to its unique profile of benzylisoquinoline alkaloids (BIAs), which includes potentia ...

Article GUID: 37004909

Author(s): Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ;

Cytokinesis is required to physically separate the daughter cells at the end of mitosis. This crucial process requires the assembly and ingression of an actomyosin ring, which must occur with high fidelity to avoid aneuploidy and cell fate changes. Most of ...

Article GUID: 36416720

Author(s): Bean BDM; Whiteway M; Martin VJJ;

The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-bas ...

Article GUID: 35708612

Author(s): Bean BDM; Mulvihill CJ; Garge RK; Boutz DR; Rousseau O; Floyd BM; Cheney W; Gardner EC; Ellington AD; Marcotte EM; Gollihar JD; Whiteway M; ...

The yeast Saccharomyces cerevisiae is powerful for studying human G protein-coupled receptors as they can be coupled to its mating pathway. However, some receptors, including the mu opioid receptor ...

Article GUID: 35610225

Author(s): Nasr MA; Timmins LR; Martin VJJ; Kwan DH;

Allosteric transcription factor (aTF) biosensors are valuable tools for engineering microbes toward a multitude of applications in metabolic engineering, biotechnology, and synthetic biology. One of the challenges toward constructing functional and diverse ...

Article GUID: 35316041