Author(s): Plissonneau C; Tam BT; Garneau PY; Morais JA; Piekny A; Santosa S;

Transwell co-culture systems are models for examining cellular crosstalk, but the impact of porous membrane material on myogenic outcomes remains uncharacterized. We investigated the influence of transwell membrane material on myoblast differentiation in co-culture with human primary adipocytes and in varying concentrations of insulin-transferrin-selenium ...

Article GUID: 42260998

Author(s): Delaney KZ; Nadeem MI; Plissonneau C; Khor N; Delaney J; Morais JA; Tsoukas MA; Pescarus R; Garneau PY; Santosa S;

Adipose tissue (AT) immune cells are implicated in the pathology of type 2 diabetes (T2D), and their content likely differs between abdominal subcutaneous adipose tissue (abSAT), femoral SAT (fmSAT), and visceral adipose tissue (VAT). Therefore, the objective of the current study was to develop a ...

Article GUID: 42255513

Author(s): Murphy J; Morais JA; Tsoukas MA; Cooke AB; Daskalopoulou SS; Santosa S;

Introduction: Adipose tissue inflammation, driven in part by immune cells, may contribute to the elevated type 2 diabetes risk in adults with childhood-onset obesity (CO) compared to those with adult-onset obesity (AO). Weight loss can modify adipose tissue immune cell composition, but whether these changes differ by obesity onset remains unknown. Method ...

Article GUID: 40831565

Author(s): Feola S; Al-Nabelsi L; Tam BT; Near J; Morais JA; Santosa S;

Objective: Compared to adulthood-onset obesity (AO), those with childhood-onset obesity (CO) are at greater risk of metabolic disease. However, the differences between these two obesity phenotypes are not clear. The aim of this study is to investigate how the age of obesity onset (CO vs. AO) affects the use of intramyocellular (IMCL) and extramyocellular ...

Article GUID: 39875595

Author(s): Murphy J; Dera A; Morais JA; Tsoukas MA; Khor N; Sazonova T; Almeida LG; Cooke AB; Daskalopoulou SS; Tam BT; Santosa S;

Objective: We aimed to examine the effect of age of obesity onset, sex, and their interaction on abdominal and femoral subcutaneous adipose tissue (SAT) morphology (degree of adipocyte hyperplasia or hypertrophy). Methods: In this cross-sectional study, we isolated adipocytes via collagenase dig ...

Article GUID: 39045668

Author(s): Murphy J; Tam BT; Kirkland JL; Tchkonia T; Giorgadze N; Pirtskhalava T; Tsoukas MA; Morais JA; Santosa S;

Objective: The aim of this study was to determine the effect of age of obesity onset on senescence-related markers in abdominal (AB) and femoral (FEM) subcutaneous adipose tissue (SAT) before and after moderate (~10%) weight loss. Methods: AB and FEM SAT were collected from human females with ch ...

Article GUID: 37194560

Author(s): Wang C; Murphy J; Delaney KZ; Khor N; Morais JA; Tsoukas MA; Lowry DE; Mutch DM; Santosa S;

Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study ...

Article GUID: 33595419

Author(s): Murphy J; Delaney KZ; Dam V; Tam BT; Khor N; Tsoukas MA; Morais JA; Santosa S;

Objective: The inflammatory environment in lower-body subcutaneous adipose tissue (SAT) has been largely unexplored. This study aimed to examine the effects of region (upper body vs. lower body) and sex on SAT immune cell profiles in young adults with obesity. Methods: Abdominal (AB) and femoral (FEM) SAT was collected from 12 males (mean [SEM] age = 30. ...

Article GUID: 33179451

Author(s): Parrott MD; Carmichael PH; Laurin D; Greenwood CE; Anderson ND; Ferland G; Gaudreau P; Belleville S; Morais JA; Kergoat MJ; Fiocco AJ;

Objective: This study examined the effect of dietary patterns and engagement in cognitive stimulating lifestyle behaviours on trajectory of global cognition, executive function (EF), and verbal episodic memory (VEM). Method: Western and prudent dietary patterns were empirically derived using foo ...

Article GUID: 33063101

Author(s): Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the gold standard method for immune cell quantification. However, quantification of immune cells from a ...

Article GUID: 32926866

Author(s): Tam BT, Murphy J, Khor N, Morais JA, Santosa S

Although childhood-onset obesity (CO) and adult-onset obesity (AO) are known to lead to distinctive clinical manifestations and disease risks, the fundamental differences between them are largely unclear. The aim of the current study is to investigate the fundamental differences between subcutaneous adipose tissue from CO and AO and identify metabolic dif ...

Article GUID: 32808657