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Functional and structural characterization of an IclR family transcription factor for the development of dicarboxylic acid biosensors

Author(s): Pham C; Nasr MA; Skarina T; Di Leo R; Kwan DH; Martin VJJ; Stogios PJ; Mahadevan R; Savchenko A; ...

Prokaryotic transcription factors (TFs) regulate gene expression in response to small molecules, thus representing promising candidates as versatile small molecule-detecting biosensors valuable for ...

Article GUID: 38696354


Divergent directed evolution of a TetR-type repressor towards aromatic molecules

Author(s): Nasr MA; Martin VJJ; Kwan DH;

Reprogramming cellular behaviour is one of the hallmarks of synthetic biology. To this end, prokaryotic allosteric transcription factors (aTF) have been repurposed as versatile tools for processing small molecule signals into cellular responses. Expanding t ...

Article GUID: 37377432


A Synthetic Biosensor for Detecting Putrescine in Beef Samples

Author(s): Selim AS; Perry JM; Nasr MA; Pimprikar JM; Shih SCC;

Biogenic amines (BAs) are toxicological risks present in many food products. Putrescine is the most common foodborne BA and is frequently used as a quality control marker. Currently, there is a lack of regulation concerning safe putrescine limits in food as ...

Article GUID: 36356104


A Versatile Transcription Factor Biosensor System Responsive to Multiple Aromatic and Indole Inducers

Author(s): Nasr MA; Timmins LR; Martin VJJ; Kwan DH;

Allosteric transcription factor (aTF) biosensors are valuable tools for engineering microbes toward a multitude of applications in metabolic engineering, biotechnology, and synthetic biology. One of the challenges toward constructing functional and diverse ...

Article GUID: 35316041


A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host.

Author(s): Harding CM, Nasr MA, Scott NE, Goyette-Desjardins G, Nothaft H, Mayer AE, Chavez SM, Huynh JP, Kinsella RL, Szymanski CM, Stallings CL, Segu ...

Nat Commun. 2019 02 21;10(1):891 Authors: Harding CM, Nasr MA, Scott NE, Goyette-Desjardins G, Nothaft H, Mayer AE, Chavez SM, Huynh JP, Kinsella RL, Szymanski CM, Stallings CL, Segura M, Feldman MF

Article GUID: 30792408


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