Author(s): Pontarelli A; Wilds CJ;

The clinical success of the antisense approach for the treatment of genetic disorders is indisputably the result of chemical modifications along the oligonucleotide (ON) scaffold, which impart desirable properties including high RNA affinity, nuclease stabi ...

Article GUID: 37667655

Author(s): Pontarelli A; Wilds CJ;

The introduction of chemical modifications on the nucleic acid scaffold has allowed for the progress of antisense oligonucleotides (ASOs) in the clinic for the treatment of a variety of disorders. In contribution to the repertoire of gene-silencing nucleic ...

Article GUID: 36857293

Author(s): Pontarelli A; Liu JT; Oh JK; Wilds CJ;

The protocols described in this article provide details regarding the synthesis and characterization of a disulfide containing linker phosphoramidite for terminal functionalization of synthetic oligonucleotides. The linker is first synthesized from 6-mercap ...

Article GUID: 36840706

Author(s): Pontarelli A; Liu JT; Movasat H; Ménard S; Oh JK; Wilds CJ;

The synthesis and incorporation of a tosylated phosphoramidite linker containing a disulfide bond is described. Incorporation of the linker into short DNA and RNA oligomers proceeded efficiently using automated solid phase synthesis. Treatment of the suppor ...

Article GUID: 35863757

Author(s): Pontarelli A; Wilds CJ;

The promise of the antisense approach to treat a variety of diseases with oligonucleotides and solutions to challenges that have been encountered in their development is attributable to chemical modification of the nucleic acid scaffold. Herein, we describe ...

Article GUID: 35452799

Author(s): Copp W, Pontarelli A, Wilds CJ

The chemical and self-assembly properties of nucleic acids make them ideal for the construction of discrete structures and stimuli-responsive devices for a diverse array of applications. Amongst the various three-dimensional assemblies, DNA tetrahedra are o ...

Article GUID: 33506614