Author(s): Ahmadi F; Tran H; Letourneau N; Little SR; Fortin A; Moraitis AN; Shih SCC;

Monoclonal antibody (mAb) discovery plays a prominent role in diagnostic and therapeutic applications. Droplet microfluidics has become a standard technology for high-throughput screening of antibody-producing cells due to high droplet single-cell confineme ...

Article GUID: 38441226

Author(s): Kékedy-Nagy L; Perry JM; Little SR; Llorens OY; Shih SCC;

We present a novel "on-off", cost-effective, rapid electrochemical aptasensor combined with a microfluidics cartridge system for the detection of ?9-THC (?9-tetrahydrocannabinol) in human saliva via differential pulse voltammetry. The assay relied on the co ...

Article GUID: 36549107

Author(s): Samlali K; Alves CL; Jezernik M; Shih SCC;

Fungal cell-wall-degrading enzymes have great utility in the agricultural and food industries. These cell-wall-degrading enzymes are known to have functions that can help defend against pathogenic organisms. The existing methods used to discover these enzym ...

Article GUID: 36438986

Author(s): Ahmadi F; Simchi M; Perry JM; Frenette S; Benali H; Soucy JP; Massarweh G; Shih SCC;

Digital microfluidics (DMF) has the signatures of an ideal liquid handling platform - as shown through almost two decades of automated biological and chemical assays. However, in the current state of DMF, we are still limited by the number of parallel biolo ...

Article GUID: 36416045

Author(s): Selim AS; Perry JM; Nasr MA; Pimprikar JM; Shih SCC;

Biogenic amines (BAs) are toxicological risks present in many food products. Putrescine is the most common foodborne BA and is frequently used as a quality control marker. Currently, there is a lack of regulation concerning safe putrescine limits in food as ...

Article GUID: 36356104

Author(s): Quach ABV; Little SR; Shih SCC;

Viral-based systems are a popular delivery method for introducing exogenous genetic material into mammalian cells. Unfortunately, the preparation of lentiviruses containing the machinery to edit the cells is labor-intensive, with steps requiring optimizatio ...

Article GUID: 35192339

Author(s): Davis AN; Samlali K; Kapadia JB; Perreault J; Shih SCC; Kharma N;

In this paper, we describe the design and performance of two digital microfluidics (DMF) chips capable of executing multiple ribozymatic reactions, with proper controls, in response to short single-stranded DNA inducers. Since the fluorescence output of a r ...

Article GUID: 34514224

Author(s): Perry JM; Soffer G; Jain R; Shih SCC;

DNA assembly and transformation are crucial to the building process in synthetic biology. These steps are significant roadblocks when engineering increasingly complex biological systems. To address this, recent development of widespread 'biofoundry' ...

Article GUID: 34369550

Author(s): Soffer G, Perry JM, Shih SCC

Optimization of engineered biological systems requires precise control over the rates and timing of gene expression. Optogenetics is used to dynamically control gene expression as an alternative to conventional chemical-based methods since it provides a mor ...

Article GUID: 33543619

Author(s): Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Small. 2020 Jul 23;:e2002400 Authors: Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Article GUID: 32705796

Author(s): Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

ACS Synth Biol. 2018 03 16;7(3):933-944 Authors: Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

Article GUID: 29516725