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Metabolomics 2023 workshop report: moving toward consensus on best QA/QC practices in LC-MS-based untargeted metabolomics

Author(s): Mosley JD; Dunn WB; Kuligowski J; Lewis MR; Monge ME; Ulmer Holland C; Vuckovic D; Zanetti KA; Schock TB; ...

Introduction: During the Metabolomics 2023 conference, the Metabolomics Quality Assurance and Quality Control Consortium (mQACC) presented a QA/QC workshop for LC-MS-based untargeted metabolomics. ...

Article GUID: 38980450


Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics

Author(s): Mosley JD; Schock TB; Beecher CW; Dunn WB; Kuligowski J; Lewis MR; Theodoridis G; Ulmer Holland CZ; Vuckovic D; Wilson ID; Zanetti KA; ...

Background: Quality assurance (QA) and quality control (QC) practices are key tenets that facilitate study and data quality across all applications of untargeted metabolomics. These important pract ...

Article GUID: 38345679


Metabolomics 2022 workshop report: state of QA/QC best practices in LC-MS-based untargeted metabolomics, informed through mQACC community engagement initiatives

Author(s): Dunn WB; Kuligowski J; Lewis M; Mosley JD; Schock T; Ulmer Holland C; Zanetti KA; Vuckovic D; ...

Introduction: The Metabolomics Quality Assurance and Quality Control Consortium (mQACC) organized a workshop during the Metabolomics 2022 conference. Objectives: The goal of the workshop was to di ...

Article GUID: 37940740


Impact of Pollutant Ozone on the Biophysical Properties of Tear Film Lipid Layer Model Membranes

Author(s): Keramatnejad M; DeWolf C;

Ozone exposure from environmental smog has been implicated as a risk factor for developing dry eye disease (DED). The tear film lipid layer (TFLL), which is the outermost layer of the tear film and responsible for surface tension reduction while blinking, i ...

Article GUID: 36837668


New metabolic signature for Chagas disease reveals sex steroid perturbation in humans and mice

Author(s): Golizeh M; Nam J; Chatelain E; Jackson Y; Ohlund LB; Rasoolizadeh A; Camargo FV; Mahrouche L; Furtos A; Sleno L; Ndao M; ...

The causative agent of Chagas disease (CD), Trypanosoma cruzi, claims thousands of lives each year. Current diagnostic tools are insufficient to ensure parasitological detection in chronically infe ...

Article GUID: 36590505


Detection of Fusobacterium nucleatum subspecies in the saliva of pre-colorectal cancer patients, using tandem mass spectrometry

Author(s): Morsi H; Golizeh M; Brosseau N; Janati AI; Emami E; Ndao M; Tran SD;

Objective: Rising evidence links Fusobacterium nucleatum (F. nucleatum) with its four subspecies; nucleatum, polymorphum, animalis, and vincentii, with the development of colorectal cancer (CRC) and its precursor colorectal adenoma (CRA). This study aims to ...

Article GUID: 34929558


A threshold LC-MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device

Author(s): Desharnais B; Lajoie MJ; Laquerre J; Mireault P; Skinner CD;

A study of impaired driving rates in the province of Québec is currently planned following the legalization of recreational cannabis in Canada. Oral fluid (OF) samples are to be collected with a Quantisal® device and sent to the laboratory for analysis. In ...

Article GUID: 33035929


Comparison of N-ethyl maleimide and N-(1-phenylethyl) maleimide for derivatization of biological thiols using liquid chromatography-mass spectrometry

Author(s): Russo MST; Napylov A; Paquet A; Vuckovic D;

The ratio between reduced and oxidized thiols, mainly glutathione and oxidized glutathione, is one of the biomarkers for the evaluation of oxidative stress. The accurate measurement of thiol concentrations is challenging because reduced thiols are easily ox ...

Article GUID: 32016570


Dexamethasone-Induced Perturbations in Tissue Metabolomics Revealed by Chemical Isotope Labeling LC-MS analysis

Author(s): Dahabiyeh LA; Malkawi AK; Wang X; Colak D; Mujamammi AH; Sabi EM; Li L; Dasouki M; Abdel Rahman AM; ...

Dexamethasone (Dex) is a synthetic glucocorticoid (GC) drug commonly used clinically for the treatment of several inflammatory and immune-mediated diseases. Despite its broad range of indications, ...

Article GUID: 31973046


In Vivo Solid-Phase Microextraction for Sampling of Oxylipins in Brain of Awake, Moving Rats

Author(s): Napylov A; Reyes-Garces N; Gomez-Rios G; Olkowicz M; Lendor S; Monnin C; Bojko B; Hamani C; Pawliszyn J; Vuckovic D; ...

Oxylipins are key lipid mediators of important brain processes, including pain, sleep, oxidative stress, and inflammation. For the first time, an in-depth profile of up to 52 oxylipins can be obtai ...

Article GUID: 31697450


Comparison of underivatized silica and zwitterionic sulfobetaine hydrophilic interaction liquid chromatography stationary phases for global metabolomics of human plasma

Author(s): Sonnenberg RA; Naz S; Cougnaud L; Vuckovic D;

To increase metabolome coverage in global LC-MS metabolomics, often both reversed-phase liquid chromatography (RPLC) and hydrophilic-interaction liquid chromatography (HILIC) are implemented in parallel. However, there is a lack of consensus in the literatu ...

Article GUID: 31439439


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