Author(s): Husser MC; Pham NP; Law C; Araujo FRB; Martin VJJ; Piekny A;
Endogenous tags have become invaluable tools to visualize and study native proteins in live cells. However, generating human cell lines carrying endogenous tags is difficult due to the low efficiency of homology-directed repair. Recently, an engineered spli ...
Article GUID: 38652106
Author(s): Singh V; Raheja Y; Basotra N; Sharma G; Tsang A; Chadha BS;
Background: The filamentous fungus Rasamsonia emersonii has immense potential to produce biorefinery relevant thermostable cellulase and hemicellulase enzymes using lignocellulosic biomass. Previously in our lab, a hyper-cellulase producing strain of R. eme ...
Article GUID: 37658430
Author(s): Dykstra CB; Pyne ME; Martin VJJ;
A fundamental challenge of metabolic engineering involves assembling and screening vast combinations of orthologous enzymes across a multistep biochemical pathway. Current pathway assembly workflows involve combining genetic parts ex vivo and assembling one ...
Article GUID: 37584634
Author(s): Abdullah M; Greco BM; Laurent JM; Garge RK; Boutz DR; Vandeloo M; Marcotte EM; Kachroo AH; ...
A major challenge to rationally building multi-gene processes in yeast arises due to the combinatorics of combining all of the individual edits into the same strain. Here, we present a precise and ...
Article GUID: 37323580
Author(s): Husser MC; Ozugergin I; Resta T; Martin VJJ; Piekny AJ;
Cytokinesis is required to physically separate the daughter cells at the end of mitosis. This crucial process requires the assembly and ingression of an actomyosin ring, which must occur with high fidelity to avoid aneuploidy and cell fate changes. Most of ...
Article GUID: 36416720
Author(s): Bean BDM; Whiteway M; Martin VJJ;
The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-bas ...
Article GUID: 35708612
Author(s): Kun RS; Garrigues S; Di Falco M; Tsang A; de Vries RP;
Aspergillus niger is a filamentous fungus well known for its ability to produce a wide variety of pectinolytic enzymes, which have many applications in the industry. The transcriptional activator GaaR is induced by 2-keto-3-deoxy-L-galactonate, a compound d ...
Article GUID: 34236481
Author(s): Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; ...
Previously, DNA microarrays analysis showed that, in co-culture with Bacillus subtilis, a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of Aspergillus niger is downregu ...
Article GUID: 34064722
Author(s): Baker PL, Orf GS, Kevershan K, Pyne ME, Bicer T, Redding KE
Appl Environ Microbiol. 2019 Sep 20;: Authors: Baker PL, Orf GS, Kevershan K, Pyne ME, Bicer T, Redding KE
Article GUID: 31540988
Author(s): Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM
Bio Protoc. 2018 Mar 20;8(6): Authors: Akhmetov A, Laurent JM, Gollihar J, Gardner EC, Garge RK, Ellington AD, Kachroo AH, Marcotte EM
Article GUID: 29770349
Author(s): Bourgeois L, Pyne ME, Martin VJJ
ACS Synth Biol. 2018 Nov 16;7(11):2675-2685 Authors: Bourgeois L, Pyne ME, Martin VJJ
Article GUID: 30372609
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