1 Department of Applied Physics, Université de Genève, 22 chemin de Pinchat, CH-1211 Genève 4, Switzerland.
2 Department of Chemistry and Biochemistry and Centre for NanoScience Research, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada.
3 Univ. Savoie Mont Blanc, SYMME, F-74000 Annecy, France.
4 OncoTheis Sàrl, 18 chemin des Aulx, CH-1228, Plan-les-Ouates, Geneva, Switzerland.
5 Department of Pharmacology and Toxicology, University of Lausanne, CH-1015, Lausanne, Switzerland.
6 Wheaton College, 26 East Main Street, Norton, Massachusetts 02766, United States.
7 Institute of Biomedical Optics University of Luebeck, Peter-Monnik-Weg 4, 23562 Luebeck, Germany.

Wavelength-Selective Nonlinear Imaging and Photo-Induced Cell Damage by Dielectric Harmonic Nanoparticles.

ACS Nano. 2020 Apr 16;:

Authors: Kilin V, Campargue G, Fureraj I, Sakong S, Sabri T, Riporto F, Vieren A, Mugnier Y, Mas C, Staedler D, Collins JM, Bonacina L, Vogel A, Capobianco JA, Wolf JP

Abstract

We introduce a nonlinear all-optical theranostics protocol based on the excitation wavelength decoupling between imaging and photoinduced damage of human cancer cells labeled by bismuth ferrite (BFO) harmonic nanoparticles (HNPs). To characterize the damage process, we rely on a scheme for in situ temperature monitoring based on upconversion nanoparticles: by spectrally resolving the emission of silica coated NaGdF4:Yb3+/Er3+ nanoparticles in close vicinity of a BFO HNP, we show that the photointeraction upon NIR-I excitation at high irradiance is associated with a temperature increase >100 °C. The observed laser-cell interaction implies a permanent change of the BFO nonlinear optical properties, which can be used as a proxy to read out the outcome of a theranostics procedure combining imaging at 980 nm and selective cell damage at 830 nm. The approach has potential applications to monitor and treat lesions within NIR light penetration depth in tissues.

PMID: 32282184 [PubMed - as supplied by publisher]