PERFORM Publications

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Title		Authors	PubMed ID
1	A review on microfluidic-assisted nanoparticle synthesis, and their applications using multiscale simulation methods	Agha A; Waheed W; Stiharu I; Nerguizian V; Destgeer G; Abu-Nada E; Alazzam A;	36800044 CONCORDIA
2	Numerical and Experimental Validation of Mixing Efficiency in Periodic Disturbance Mixers	López RR; Sánchez LM; Alazzam A; Burnier JV; Stiharu I; Nerguizian V;	34577745 ENCS
3	Surface Response Based Modeling of Liposome Characteristics in a Periodic Disturbance Mixer.	López RR, Ocampo I, Sánchez LM, Alazzam A, Bergeron KF, Camacho-León S, Mounier C, Stiharu I, Nerguizian V	32106424 ENCS
4	Dielectrophoresis Multipath Focusing of Microparticles through Perforated Electrodes in Microfluidic Channels.	Alazzam A, Al-Khaleel M, Riahi MK, Mathew B, Gawanmeh A, Nerguizian V	31394810 ENCS
5	Fabrication of Porous Gold Film Using Graphene Oxide as a Sacrificial Layer.	Alazzam A, Alamoodi N, Abutayeh M, Stiharu I, Nerguizian V	31323903 ENCS
6	Lateral fluid flow fractionation using dielectrophoresis (LFFF-DEP) for size-independent, label-free isolation of circulating tumor cells.	Waheed W, Alazzam A, Mathew B, Christoforou N, Abu-Nada E	29734073 ENCS
7	The effect of dielectrophoresis on living cells: crossover frequencies and deregulation in gene expression.	Nerguizian V, Stiharu I, Al-Azzam N, Yassine-Diab B, Alazzam A	31099354 ENCS

Title:	The effect of dielectrophoresis on living cells: crossover frequencies and deregulation in gene expression.
Authors:	Nerguizian V, Stiharu I, Al-Azzam N, Yassine-Diab B, Alazzam A
Link:	https://www.ncbi.nlm.nih.gov/pubmed/31099354?dopt=Abstract
Publication:
Keywords:
PMID:	31099354	Category:	Analyst	Date Added:	2019-06-04
Dept Affiliation:	ENCS 1 Department of Electrical Engineering, École de technologie supérieure (ÉTS), Montreal, Canada. 2 Department of Mechanical and Industrial Engineering, Concordia University, Montreal, Canada. 3 Department of Physiology and Biochemistry, Jordan University of Science and Technology, Irbid, Jordan. 4 Caprion Biosciences, Montreal, Quebec, Canada. 5 Department of Mechanical Engineering, Khalifa University, Abu Dhabi, United Arab Emirates. anas.alazzam@ku.ac.ae and Department of Electrical Engineering, École de technologie supérieure (ÉTS), Montreal, Canada.

Description:

The effect of dielectrophoresis on living cells: crossover frequencies and deregulation in gene expression.

Analyst. 2019 May 17;:

Authors: Nerguizian V, Stiharu I, Al-Azzam N, Yassine-Diab B, Alazzam A

Abstract

This article documents the effect of dielectrophoresis on living cells. Given the longer duration procedures performed on microfluidic platforms, the influence of electric fields of high intensity may be of interest in manipulations other than dielectrophoresis. The crossover frequencies of several cell lines were experimentally determined using a microfluidic device. The crossover frequencies are investigated at different medium conductivities for red blood cells, white blood cells-Jurkat, 92.1 and OCM melanoma, and MDA-MB-231 breast cancer cell lines. The effect of dielectrophoresis on the cells at the gene level was also investigated by studying the alteration in gene expressions using microarray analysis. The alterations in genes due to the manipulation of cells at 10 kHz and 100 kHz with a sinusoidal 10 V peak signal for 60 minutes are explored. The two frequencies correspond to negative and positive dielectrophoresis, respectively. The cell line MDA-MB-231 is used as a model for studying the genes in this work. The dielectrophoresis was found to alter genes related to apoptosis, rRNA transcription, cellular respiration, energy production, cellular transcriptional activity, and other cellular functions.

PMID: 31099354 [PubMed - as supplied by publisher]

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