Keyword search (4,164 papers available)

"Benoit-Gelber I" Authored Publications:

Title Authors PubMed ID
1 Global survey of secondary metabolism in em Aspergillus niger /em via activation of specific transcription factors Semper C; Pham TTM; Ram S; Palys S; Evdokias G; Ouedraogo JP; Moisan MC; Geoffrion N; Reid I; Di Falco M; Bailey Z; Tsang A; Benoit-Gelber I; Savchenko A; 40852424
GENOMICS
2 Identification of a Novel Biosynthetic Gene Cluster in Aspergillus niger Using Comparative Genomics Evdokias G; Semper C; Mora-Ochomogo M; Di Falco M; Nguyen TTM; Savchenko A; Tsang A; Benoit-Gelber I; 34064722
BIOLOGY
3 Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance. Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP 31674709
CSFG
4 Secretion of small proteins is species-specific within Aspergillus sp. Valette N, Benoit-Gelber I, Falco MD, Wiebenga A, de Vries RP, Gelhaye E, Morel-Rouhier M 27153937
CSFG
5 Expression-based clustering of CAZyme-encoding genes of Aspergillus niger. Gruben BS, Mäkelä MR, Kowalczyk JE, Zhou M, Benoit-Gelber I, De Vries RP 29169319
CSFG
6 Evolutionary Adaptation to Generate Mutants. de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I 29876815
BIOLOGY
7 The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum. Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP 30797054
CSFG

 

Title:Evolutionary Adaptation to Generate Mutants.
Authors:de Vries RP,  Lubbers R,  Patyshakuliyeva A,  Wiebenga A,  Benoit-Gelber I
Link:https://www.ncbi.nlm.nih.gov/pubmed/29876815?dopt=Abstract
DOI:10.1007/978-1-4939-7804-5_12
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:Adaptation;  Evolution;  Mutants;  Screening;  Selection; 
PMID:29876815 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: BIOLOGY
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands. r.devries@westerdijkinstitute.nl.
2 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
3 Centre for Structural and Functional Genomics, Department of Biology, Concordia University, Montreal, QC, Canada.

Description:

Evolutionary Adaptation to Generate Mutants.

Methods Mol Biol. 2018;1775:133-137

Authors: de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I

Abstract

In this chapter we describe a method to generate mutants of filamentous fungi using their genomic plasticity and rapid adaptability to their environment. This method is based on spontaneous mutations occurring in relation to improved growth of fungi on media by repeated inoculation resulting in adaptation of the strain to the condition. The critical aspect of this method is the design of the selective media, which will depend strongly on the phenomenon that will be studied. This method is advantageous over UV or chemical random mutagenesis as it results in a lower frequency of undesired mutations and can result in strains that combined with (post)genomic approaches can enhance our understanding of the mechanisms driving various biological processes. In addition, it can be used to obtain better strains for various industrial applications. The method described here is specific for sporulating fungi and has so far not yet been tested for nonsporulating fungi.

PMID: 29876815 [PubMed - indexed for MEDLINE]




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