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PubMed Publications| Keyword search (4,163 papers available) |  |
"Little SR" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Correction: Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution | Patel MA; Boribong BP; Sinha H; Xiao B; Xie K; Vo PQN; Chin AB; Ellouzi A; Little SR; Shih SCC; Wu H; Muller WJ; Hirukawa A; | 41028230 BIOLOGY |
| 2 | Miniaturized scalable arrayed CRISPR screening in primary cells enables discovery at the single donor resolution | Patel MA; Boribong BP; Sinha H; Xiao B; Xie K; Vo PQN; Chin AB; Ellouzi A; Little SR; Shih S; Wu H; Muller WJ; Hirukawa A; | 40790054 BIOLOGY |
| 3 | Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells | Gholizadeh F; Hajiaghayi M; Choi JS; Little SR; Rahbari N; Kargar M; Brotto K; Han E; Shih SCC; Darlington PJ; | 40405417 BIOLOGY |
| 4 | A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies | Little SR; Rahbari N; Hajiaghayi M; Gholizadeh F; Cloarec-Ung FM; Phillips J; Sinha H; Hirukawa A; Knapp DJHF; Darlington PJ; Shih SCC; | 40390294 BIOLOGY |
| 5 | The β2-adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner | Hajiaghayi M; Gholizadeh F; Han E; Little SR; Rahbari N; Ardila I; Lopez Naranjo C; Tehranimeh K; Shih SCC; Darlington PJ; | 39445009 BIOLOGY |
| 6 | An Automated Single-Cell Droplet-Digital Microfluidic Platform for Monoclonal Antibody Discovery | Ahmadi F; Tran H; Letourneau N; Little SR; Fortin A; Moraitis AN; Shih SCC; | 38441226 BIOLOGY |
| 7 | An electrochemical aptasensor for Δ9-tetrahydrocannabinol detection in saliva on a microfluidic platform | Kékedy-Nagy L; Perry JM; Little SR; Llorens OY; Shih SCC; | 36549107 BIOLOGY |
| 8 | Viral Generation, Packaging, and Transduction on a Digital Microfluidic Platform | Quach ABV; Little SR; Shih SCC; | 35192339 BIOLOGY |
| Title: | Viral Generation, Packaging, and Transduction on a Digital Microfluidic Platform | ||||
| Authors: | Quach ABV, Little SR, Shih SCC | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/35192339/ | ||||
| DOI: | 10.1021/acs.analchem.1c05227 | ||||
| Publication: | Analytical chemistry | ||||
| Keywords: | |||||
| PMID: | 35192339 | Category: | Date Added: | 2022-02-22 | |
| Dept Affiliation: |
BIOLOGY
1 Department of Biology, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6, Canada. 2 Centre for Applied Synthetic Biology, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6, Canada. 3 Department of Electrical and Computer Engineering, Concordia University, 1455 de Maisonneuve Blvd. West, Montréal, Québec H3G 1M8, Canada. |
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Description: |
Viral-based systems are a popular delivery method for introducing exogenous genetic material into mammalian cells. Unfortunately, the preparation of lentiviruses containing the machinery to edit the cells is labor-intensive, with steps requiring optimization and sensitive handling. To mitigate these challenges, we introduce the first microfluidic method that integrates lentiviral generation, packaging, and transduction. The new method allows the production of viral titers between 106 and 107 (similar to macroscale production) and high transduction efficiency for hard-to-transfect cell lines. We extend the technique for gene editing applications and show how this technique can be used to knock out and knock down estrogen receptor gene-a gene prominently responsible for 70% of breast cancer cases. This new technique is automated with multiplexing capabilities, which have the potential to standardize the methods for viral-based genome engineering. |
