PERFORM Publications

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Title		Authors	PubMed ID
1	The age of obesity onset affects changes in subcutaneous adipose tissue macrophages and T cells after weight loss	Murphy J; Morais JA; Tsoukas MA; Cooke AB; Daskalopoulou SS; Santosa S;	40831565 SOH
2	Age of obesity onset affects subcutaneous adipose tissue cellularity differently in the abdominal and femoral region	Murphy J; Dera A; Morais JA; Tsoukas MA; Khor N; Sazonova T; Almeida LG; Cooke AB; Daskalopoulou SS; Tam BT; Santosa S;	39045668 SOH
3	Senescence markers in subcutaneous preadipocytes differ in childhood- versus adult-onset obesity before and after weight loss	Murphy J; Tam BT; Kirkland JL; Tchkonia T; Giorgadze N; Pirtskhalava T; Tsoukas MA; Morais JA; Santosa S;	37194560 PERFORM
4	Body-composition phenotypes and their associations with cardiometabolic risks and health behaviours in a representative general US sample	Kakinami L; Plummer S; Cohen TR; Santosa S; Murphy J;	36183799 PERFORM
5	Adiposity and muscle mass phenotyping is not superior to BMI in detecting cardiometabolic risk in a cross-sectional study	Kakinami L; Danieles PK; Ajibade K; Santosa S; Murphy J;	34231966 PERFORM
6	Altered immunometabolism in adipose tissue: a major contributor to the ageing process?	Delaney KZ; Gillespie ZE; Murphy J; Wang C;	34159597 PERFORM
7	Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity	Wang C; Murphy J; Delaney KZ; Khor N; Morais JA; Tsoukas MA; Lowry DE; Mutch DM; Santosa S;	33595419 PERFORM
8	Sex Affects Regional Variations in Subcutaneous Adipose Tissue T Cells but not Macrophages in Adults with Obesity	Murphy J; Delaney KZ; Dam V; Tam BT; Khor N; Tsoukas MA; Morais JA; Santosa S;	33179451 PERFORM
9	Methodological considerations for the measurement of arterial stiffness using applanation tonometry	Cooke AB; Kuate Defo A; Dasgupta K; Papaioannou TG; Lee J; Morin SN; Murphy J; Santosa S; Daskalopoulou SS;	33031179 PERFORM
10	A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.	Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S	32926866 PERFORM
11	Acetyl-CoA regulation, OXPHOS integrity and leptin level are different in females with different onsets of obesity.	Tam BT, Murphy J, Khor N, Morais JA, Santosa S	32808657 PERFORM
12	Intra-Abdominal Adipose Tissue Quantification by Alternative Versus Reference Methods: A Systematic Review and Meta-Analysis.	Murphy J, Bacon SL, Morais JA, Tsoukas MA, Santosa S	31131996 PERFORM
13	Factors associated with adipocyte size reduction after weight loss interventions for overweight and obesity: a systematic review and meta-regression.	Murphy J, Moullec G, Santosa S	28081776 PERFORM

Title:	A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.
Authors:	Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S
Link:	https://www.ncbi.nlm.nih.gov/pubmed/32926866
DOI:	10.1016/j.ab.2020.113951
Publication:	Analytical biochemistry
Keywords:	Adipose tissue; Flow cytometry; Humans; Immune cells; Inflammation; Obesity;
PMID:	32926866	Category:	Anal Biochem	Date Added:	2020-09-15
Dept Affiliation:	PERFORM 1 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada. 2 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Division of Geriatric Medicine, McGill University Health Centre - Royal Victoria Hospital, 1001, Décarie, Montreal, Québec, H4A 3J1, Canada. 3 Departement Du Chirurgie, Hôpital Du Sacré-Coeur de Montréal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada. 4 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada. Electronic address: s.santosa@concordia.ca.

Description:

A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.

Anal Biochem. 2020 Sep 11; :113951

Authors: Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S

Abstract

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the gold standard method for immune cell quantification. However, quantification of immune cells from adipose tissue presents a number of challenges because of the complexities of working with an oily substance and the rapid deterioration of immune cell viability before analysis can be performed. Here we present a highly reproducible flow cytometry protocol for the quantification of immune cells in human adipose tissue, which overcomes these issues.

PMID: 32926866 [PubMed - as supplied by publisher]

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