Keyword search (4,163 papers available)

"O'Flaherty DK" Authored Publications:

Title Authors PubMed ID
1 O4-alkyl-2'-deoxythymidine cross-linked DNA to probe recognition and repair by O6-alkylguanine DNA alkyltransferases. McManus FP, O'Flaherty DK, Noronha AM, Wilds CJ 22850722
CHEMBIOCHEM
2 Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA. O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ 26624500
CHEMBIOCHEM
3 O(6)-Alkylguanine DNA Alkyltransferase Repair Activity Towards Intrastrand Cross-Linked DNA is Influenced by the Internucleotide Linkage. O'Flaherty DK, Wilds CJ 26692563
CHEMISTRY
4 Lesion Orientation of O4-Alkylthymidine Influences Replication by Human DNA Polymerase η. O'Flaherty DK, Patra A, Su Y, Guengerich FP, Egli M, Wilds CJ 27574558
CHEMBIOCHEM
5 Preparation of Intrastrand {G}O(6) -Alkylene-O(6) {G} Cross-Linked Oligonucleotides. O'Flaherty DK, Wilds CJ 27584704
CHEMBIOCHEM
6 O6-2'-Deoxyguanosine-butylene-O6-2'-deoxyguanosine DNA Interstrand Cross-Links Are Replication-Blocking and Mutagenic DNA Lesions. Xu W, Kool D, O'Flaherty DK, Keating AM, Sacre L, Egli M, Noronha A, Wilds CJ, Zhao L 27768841
CHEMBIOCHEM
7 Site-specific covalent capture of human O6-alkylguanine-DNA-alkyltransferase using single-stranded intrastrand cross-linked DNA. O'Flaherty DK, Wilds CJ 27886318
CHEMBIOCHEM
8 Structural basis of interstrand cross-link repair by O6-alkylguanine DNA alkyltransferase. Denisov AY, McManus FP, O'Flaherty DK, Noronha AM, Wilds CJ 28937154
CHEMBIOCHEM
9 AGT Activity Towards Intrastrand Crosslinked DNA is Modulated by the Alkylene Linker. O'Flaherty DK, Wilds CJ 28980757
CHEMBIOCHEM
10 Altering Residue 134 Confers an Increased Substrate Range of Alkylated Nucleosides to the E. coli OGT Protein. Schoonhoven NM, O'Flaherty DK, McManus FP, Sacre L, Noronha AM, Kornblatt MJ, Wilds CJ 29137116
CHEMBIOCHEM
11 Covalent capture of OGT's active site using engineered human-E. coli chimera and intrastrand DNA cross-links. Copp W, O'Flaherty DK, Wilds CJ 30430154
CHEMBIOCHEM

 

Title:AGT Activity Towards Intrastrand Crosslinked DNA is Modulated by the Alkylene Linker.
Authors:O'Flaherty DK,  Wilds CJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/28980757?dopt=Abstract
Publication:
Keywords:
PMID:28980757 Category:Chembiochem Date Added:2019-05-31
Dept Affiliation: CHEMBIOCHEM
1 Department of Chemistry and Biochemistry, Concordia University Montreal, 7141 Sherbrooke Street W., Montreal, Quebec, H4B 1R6, Canada.
2 Present address: Howard Hughes Medical Institute, Department of Molecular Biology and, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA, 02114, USA.

Description:

AGT Activity Towards Intrastrand Crosslinked DNA is Modulated by the Alkylene Linker.

Chembiochem. 2017 12 05;18(23):2351-2357

Authors: O'Flaherty DK, Wilds CJ

Abstract

DNA oligomers containing dimethylene and trimethylene intrastrand crosslinks (IaCLs) between the O4 and O6 atoms of neighboring thymidine (T) and 2'-deoxyguanosine (dG) residues were prepared by solid-phase synthesis. UV thermal denaturation (Tm ) experiments revealed that these IaCLs had a destabilizing effect on the DNA duplex relative to the control. Circular dichroism spectroscopy suggested these IaCLs induced minimal structural distortions. Susceptibility to dealkylation by reaction with various O6 -alkylguanine DNA alkyltransferases (AGTs) from human and Escherichia coli was evaluated. It was revealed that only human AGT displayed activity towards the IaCL DNA, with reduced efficiency as the IaCL shortened (from four to two methylene linkages). Changing the site of attachment of the ethylene linkage at the 5'-end of the IaCL to the N3 atom of T had minimal influence on duplex stability and structure, and was refractory to AGT activity.

PMID: 28980757 [PubMed - indexed for MEDLINE]




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