Keyword search (4,164 papers available)

"Peng M" Authored Publications:

Title Authors PubMed ID
1 The Sugar Metabolic Model of Aspergillus niger Can Only Be Reliably Transferred to Fungi of Its Phylum Li J; Chroumpi T; Garrigues S; Kun RS; Meng J; Salazar-Cerezo S; Aguilar-Pontes MV; Zhang Y; Tejomurthula S; Lipzen A; Ng V; Clendinen CS; Tolic N; Grigoriev IV; Tsang A; Mäkelä MR; Snel B; Peng M; de Vries RP; 36547648
BIOLOGY
2 Carbohydrate esterase family 16 contains fungal hemicellulose acetyl esterases (HAEs) with varying specificity Venegas FA; Koutaniemi S; Langeveld SMJ; Bellemare A; Chong SL; Dilokpimol A; Lowden MJ; Hilden KS; Leyva-Illades JF; Mäkelä MR; My Pham TT; Peng M; Hancock MA; Zheng Y; Tsang A; Tenkanen M; Powlowski J; de Vries RP; 35405333
CSFG
3 Penicillium subrubescens adapts its enzyme production to the composition of plant biomass. Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP 32408196
CSFG
4 Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance. Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP 31674709
CSFG
5 Glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens. Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, Mäkelä MR, de Vries RP 31585998
CSFG
6 The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger. Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ 28417461
CSFG
7 Genomic and exoproteomic diversity in plant biomass degradation approaches among Aspergilli Mäkelä MR; DiFalco M; McDonnell E; Nguyen TTM; Wiebenga A; Hildén K; Peng M; Grigoriev IV; Tsang A; de Vries RP; 30487660
CSFG
8 The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum. Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP 30797054
CSFG

 

Title:Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.
Authors:Lubbers RJMLiwanag AJPeng MDilokpimol ABenoit-Gelber Ide Vries RP
Link:https://www.ncbi.nlm.nih.gov/pubmed/31674709?dopt=Abstract
DOI:10.1111/jam.14505
Publication:Journal of applied microbiology
Keywords:Aspergillusadaptive evolutionaromatic metabolismcell factoryferulic acid tolerancemutagenesis
PMID:31674709 Category:J Appl Microbiol Date Added:2019-11-02
Dept Affiliation: CSFG
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
2 Centre for Structural and Functional Genomics, Concordia University, Montréal, Canada.

Description:

Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.

J Appl Microbiol. 2019 Nov 01;:

Authors: Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP

Abstract

AIMS: To create an Aspergillus niger mutant with increased tolerance against ferulic acid using evolutionary adaptation.

METHODS AND RESULTS: Evolutionary adaptation of Aspergillus niger N402 was performed by consecutive growth on increasing concentrations of ferulic acid in the presence of 25 mmol l-1 D-fructose, starting from 0·5 mmol l-1 and ending with 5 mmol l-1 ferulic acid. The A. niger mutant obtained after six months, named Fa6, showed increased ferulic acid tolerance compared to the parent. In addition, Fa6 has increased ferulic acid consumption and a higher conversion rate, suggesting that the mutation affects aromatic metabolism of this species. Transcriptome analysis of the evolutionary mutant on ferulic acid revealed a distinct gene expression profile compared to the wild type. Further analysis of this mutant and the parent strain provided the first experimental confirmation that A. niger converts coniferyl alcohol to ferulic acid.

CONCLUSIONS: The evolutionary adaptive A. niger mutant Fa6 has beneficial mutations that increase the tolerance, conversion rate and uptake of ferulic acid.

SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrates that evolutionary adaptation is a powerful tool to modify microorganisms towards increased tolerance to harsh conditions, which is beneficial for various industrial applications.

PMID: 31674709 [PubMed - as supplied by publisher]





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