Keyword search (4,163 papers available)

"Soffer G" Authored Publications:

Title Authors PubMed ID
1 Expanding the limits towards 'one-pot' DNA assembly and transformation on a rapid-prototype microfluidic device Perry JM; Soffer G; Jain R; Shih SCC; 34369550
BIOLOGY
2 Real-Time Optogenetics System for Controlling Gene Expression Using a Model-Based Design. Soffer G, Perry JM, Shih SCC 33543619
BIOLOGY
3 One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation. Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC 32705796
BIOLOGY
4 Integration of World-to-Chip Interfaces with Digital Microfluidics for Bacterial Transformation and Enzymatic Assays. Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC 30945840
ENCS

 

Title:One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.
Authors:Samlali K,  Ahmadi F,  Quach ABV,  Soffer G,  Shih SCC
Link:https://www.ncbi.nlm.nih.gov/pubmed/32705796
DOI:10.1002/smll.202002400
Publication:Small (Weinheim an der Bergstrasse, Germany)
Keywords:digital microfluidics;  droplet microfluidics;  gene-editing;  single-cell;  transfection; 
PMID:32705796 Category:Small Date Added:2020-07-27
Dept Affiliation: BIOLOGY
1 Department of Electrical and Computer Engineering, Concordia University, Montréal, Québec, H3G 1M8, Canada.
2 Centre for Applied Synthetic Biology, Concordia University, Montréal, Québec, H4B 1R6, Canada.
3 Department of Biology, Concordia University, Montréal, Québec, H4B 1R6, Canada.

Description:

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.

Small. 2020 Jul 23;:e2002400

Authors: Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Abstract

Generating a stable knockout cell line is a complex process that can take several months to complete. In this work, a microfluidic method that is capable of isolating single cells in droplets, selecting successful edited clones, and expansion of these isoclones is introduced. Using a hybrid microfluidics method, droplets in channels can be individually addressed using a co-planar electrode system. In the hybrid microfluidics device, it is shown that single cells can be trapped and subsequently encapsulate them on demand into pL-sized droplets. Furthermore, droplets containing single cells are either released, kept in the traps, or merged with other droplets by the application of an electric potential to the electrodes that is actuated through an in-house user interface. This high precision control is used to successfully sort and recover single isoclones to establish monoclonal cell lines, which is demonstrated with a heterozygous NCI-H1299 lung squamous cell population resulting from loss-of-function eGFP and RAF1 gene knockout transfections.

PMID: 32705796 [PubMed - as supplied by publisher]




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