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PubMed Publications| Keyword search (4,163 papers available) |  |
"Sonenberg N" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Alzheimer model chip with microglia BV2 cells | Yazdanpanah Moghadam E; Sonenberg N; Packirisamy M; | 40623989 ENCS |
| 2 | Microfluidic Wound-Healing Assay for Comparative Study on Fluid Dynamic, Chemical and Mechanical Wounding on Microglia BV2 Migration | Yazdanpanah Moghadam E; Sonenberg N; Packirisamy M; | 39203655 ENCS |
| 3 | Microfluidic Wound-Healing Assay for ECM and Microenvironment Properties on Microglia BV2 Cells Migration | Yazdanpanah Moghadam E; Sonenberg N; Packirisamy M; | 36832056 ENCS |
| 4 | Lab-On-A-Chip for the Development of Pro-/Anti-Angiogenic Nanomedicines to Treat Brain Diseases. | Subramaniyan Parimalam S, Badilescu S, Sonenberg N, Bhat R, Packirisamy M | 31817343 ENCS |
| 5 | The eIF2α Kinase GCN2 Modulates Period and Rhythmicity of the Circadian Clock by Translational Control of Atf4. | Pathak SS, Liu D, Li T, de Zavalia N, Zhu L, Li J, Karthikeyan R, Alain T, Liu AC, Storch KF, Kaufman RJ, Jin VX, Amir S, Sonenberg N, Cao R | 31522764 CSBN |
| 6 | Light-regulated translational control of circadian behavior by eIF4E phosphorylation. | Cao R, Gkogkas CG, de Zavalia N, Blum ID, Yanagiya A, Tsukumo Y, Xu H, Lee C, Storch KF, Liu AC, Amir S, Sonenberg N | 25915475 CSBN |
| 7 | mTOR signaling in VIP neurons regulates circadian clock synchrony and olfaction | Liu D; Stowie A; de Zavalia N; Leise T; Pathak SS; Drewes LR; Davidson AJ; Amir S; Sonenberg N; Cao R; | 29555746 CSBN |
| Title: | Microfluidic Wound-Healing Assay for ECM and Microenvironment Properties on Microglia BV2 Cells Migration | ||||
| Authors: | Yazdanpanah Moghadam E, Sonenberg N, Packirisamy M | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/36832056/ | ||||
| DOI: | 10.3390/bios13020290 | ||||
| Publication: | Biosensors | ||||
| Keywords: | cell migration; extracellular matrix coating; microfluidic wound-healing migration assay; microglia cells; substrate rigidity; | ||||
| PMID: | 36832056 | Category: | Date Added: | 2023-02-25 | |
| Dept Affiliation: |
ENCS
1 Optical-Bio Microsystems Laboratory, Micro-Nano-Bio Integration Center, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, QC H3G 1M8, Canada. 2 Department of Biochemistry, Goodman Cancer Research Center, McGill University, Montreal, QC H3A 1A3, Canada. |
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Description: |
Microglia cells, as the resident immune cells of the central nervous system (CNS), are highly motile and migratory in development and pathophysiological conditions. During their migration, microglia cells interact with their surroundings based on the various physical and chemical properties in the brain. Herein, a microfluidic wound-healing chip is developed to investigate microglial BV2 cell migration on the substrates coated with extracellular matrixes (ECMs) and substrates usually used for bio-applications on cell migration. In order to generate the cell-free space (wound), gravity was utilized as a driving force to flow the trypsin with the device. It was shown that, despite the scratch assay, the cell-free area was created without removing the extracellular matrix coating (fibronectin) using the microfluidic assay. It was found that the substrates coated with Poly-L-Lysine (PLL) and gelatin stimulated microglial BV2 migration, while collagen and fibronectin coatings had an inhibitory effect compared to the control conditions (uncoated glass substrate). In addition, the results showed that the polystyrene substrate induced higher cell migration than the PDMS and glass substrates. The microfluidic migration assay provides an in vitro microenvironment closer to in vivo conditions for further understanding the microglia migration mechanism in the brain, where the environment properties change under homeostatic and pathological conditions. |
