Keyword search (4,163 papers available)

"J Proteome Res" Category Publications:

Title Authors PubMed ID
1 Quantitative analysis of the yeast proteome by incorporation of isotopically labeled leucine. Jiang H, English AM 12645890
CHEMBIOCHEM
2 Evaluation of D10-Leu metabolic labeling coupled with MALDI-MS analysis in studying the response of the yeast proteome to H2O2 challenge Jiang H; English AM; 17022625
CBAMS
3 Progress and Challenges in Ocean Metaproteomics and Proposed Best Practices for Data Sharing. Saito MA, Bertrand EM, Duffy ME, Gaylord DA, Held NA, Hervey WJ, Hettich RL, Jagtap PD, Janech MG, Kinkade DB, Leary DH, McIlvin MR, Moore EK, Morris RM, Neely BA, Nunn BL, Saunders JK, Shepherd AI, Symmonds NI, Walsh DA 30702898
BIOLOGY

 

Title:Evaluation of D10-Leu metabolic labeling coupled with MALDI-MS analysis in studying the response of the yeast proteome to H2O2 challenge
Authors:Jiang HEnglish AM
Link:https://pubmed.ncbi.nlm.nih.gov/17022625/
DOI:10.1021/pr060019m
Publication:Journal of proteome research
Keywords:
PMID:17022625 Category:J Proteome Res Date Added:2019-06-19
Dept Affiliation: CBAMS
1 Centre for Biological Applications of Mass Spectrometry, Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada H4B 1R6.

Description:

An efficient D10-Leu metabolic-labeling method combined with isotope-ratio quantitation by MALDI-TOF MS was used to probe the response of the yeast proteome to H2O2. Control cultures correct for effects not associated with H2O2 challenge. A stress-response index to H2O2 (SRIH2O2) is defined, and values are reported for seven proteins at 45-225 min following exposure to 0.4 mM H2O2. The time course of protein accumulation in unstressed cells following the H10- to D10-SCD switch suggests that proteome responses at <45 min could be monitored by addition of excess D10-Leu to H10-cultures.





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