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Title		Authors	PubMed ID
1	Importin-binding mediates the intramolecular regulation of anillin during cytokinesis.	Beaudet D, Pham N, Skaik N, Piekny A	32238082 BIOLOGY
2	Hof1 plays a checkpoint related role in MMS induced DNA damage response in Candida albicans.	Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M, Goyal A, Omran RP, Mallick J, Whiteway M	31940254 BIOLOGY
3	Characterization of a novel separase-interacting protein and candidate new securin, Eip1p, in the fungal pathogen Candida albicans	Sparapani S; Bachewich C;	31411946 BIOLOGY
4	Active Ran regulates anillin function during cytokinesis.	Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A	28931593 BIOLOGY
5	The Na+(K+)/H+ exchanger Nhx1 controls multivesicular body-vacuolar lysosome fusion.	Karim MA, Brett CL	29212874 BIOLOGY
6	The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain.	Sharmeen N, Sulea T, Whiteway M, Wu C	30650049 BIOLOGY

Title:	Active Ran regulates anillin function during cytokinesis.
Authors:	Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A
Link:	https://www.ncbi.nlm.nih.gov/pubmed/28931593?dopt=Abstract
DOI:	10.1091/mbc.E17-04-0253
Publication:	Molecular biology of the cell
Keywords:
PMID:	28931593	Category:	Mol Biol Cell	Date Added:	2019-06-07
Dept Affiliation:	BIOLOGY 1 Department of Biology, Concordia University, Montreal, QC H4B 1R6, Canada. 2 Program in Cell Biology, the Hospital for Sick Children, Toronto, ON M5G 0A4, Canada. 3 Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada. 4 Centre for Microscopy and Cellular Imaging, Concordia University, Montreal, QC H4B 1R6, Canada. 5 Program in Cell Biology, the Hospital for Sick Children, Toronto, ON M5G 0A4, Canada alisa.piekny@concordia.ca.

Description:

Active Ran regulates anillin function during cytokinesis.

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531

Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Abstract

Cytokinesis cleaves a cell into two daughters at the end of mitosis, and must be spatially coordinated with chromosome segregation to prevent aneuploidy. The dogma is that the mitotic spindle governs the assembly and constriction of an actomyosin ring. Here, we reveal a function for active Ran in spatially restricting the ring. Our model is that during anaphase, "free" importins, whose gradient inversely correlates with active Ran and chromatin position, function as a molecular ruler for the recruitment and localization of anillin, a contractile protein and a crucial regulator of cytokinesis. We found that decreasing Ran-GTP levels or tethering active Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes. Anillin contains a conserved nuclear localization signal (NLS) at its C-terminus that binds to importin-ß and is required for cortical polarity and cytokinesis. Mutating the NLS decreases anillin's cortical affinity, causing it to be more dominantly regulated by microtubules. Anillin contains a RhoA-GTP binding domain, which autoinhibits the NLS and the neighboring microtubule-binding domain, and RhoA-GTP binding may relieve this inhibition during mitosis. Retention of the C-terminal NLS in anillin homologues suggests that this is a conserved mechanism for controlling anillin function.

PMID: 28931593 [PubMed - indexed for MEDLINE]

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