PERFORM Publications

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Title		Authors	PubMed ID
1	Interpersonal Capitalization and Unmet Interpersonal Needs Among Adolescents at Varying Risk for Suicidal Ideation: A Daily Diary Study	Perezmontemayor Cruz I; MacNeil S; Renaud J; Gouin JP;	41928498 PSYCHOLOGY
2	Perceived Partner Responsiveness is Associated with Longitudinal Changes in Circulating Inflammatory Biomarkers Among Caregiving Mothers in Midlife	Gouin JP; Sánchez-Carro Y; Cruz IP; MacNeil S;	41876038 PSYCHOLOGY
3	Aerobic fitness modulates arithmetic strategy use in college-aged young adults	McGowan AL; Ellison OK; Ham MS; Chandler MC; Pontifex MB;	40889823 HKAP
4	Clustering and Interpretability of Residential Electricity Demand Profiles	Kallel S; Amayri M; Bouguila N;	40218540 ENCS
5	Face Boundary Formulation for Harmonic Models: Face Image Resembling	Huang HT; Li ZC; Wei Y; Suen CY;	39852327 CONCORDIA
6	A protocol for trustworthy EEG decoding with neural networks	Borra D; Magosso E; Ravanelli M;	39549492 ENCS
7	Bulk Free Radical Terpolymerization of Butyl Acrylate, 2-Methylene-1,3-Dioxepane and Vinyl Acetate: Terpolymer Reactivity Ratio Estimation	Movafagh M; Meek KM; Scott AJ; Penlidis A; Dubé MA;	38794524 ENCS
8	Respiratory sinus arrhythmia moderates the interpersonal consequences of brooding rumination	Caldwell W; MacNeil S; Wrosch C; McGrath JJ; Dang-Vu TT; Morin AJS; Gouin JP;	36844897 HKAP
9	Atorvastatin lowers serum calcium levels in lithium-users: results from a randomized controlled trial	Soh JF; Bodenstein K; Yu OHY; Linnaranta O; Renaud S; Mahdanian A; Su CL; Mucsi I; Mulsant B; Herrmann N; Rajji T; Beaulieu S; Sekhon H; Rej S;	36153583 PSYCHOLOGY
10	Multiple Identifications of Employees in an Organization: Salience and Relationships of Foci and Dimensions	Sidorenkov AV; Borokhovski EF; Stroh WA; Naumtseva EA;	35735392 CSLP
11	Neural evidence for age-related deficits in the representation of state spaces	Ruel A; Bolenz F; Li SC; Fischer A; Eppinger B;	35510942 PERFORM
12	Engineering Yeast for De Novo Synthesis of the Insect Repellent Nepetalactone	Davies ME; Tsyplenkov D; Martin VJJ;	34748704 BIOLOGY
13	Relationships between Employees&#39, Identifications and Citizenship Behavior in Work Groups: The Role of the Regularity and Intensity of Interactions	Sidorenkov AV; Borokhovski EF;	34206317 CSLP
14	Predicting Interpersonal Outcomes From Information Processing Tasks Using Personally Relevant and Generic Stimuli: A Methodology Study	Serravalle L; Tsekova V; Ellenbogen MA;	33071861 CRDH
15	What attributes are relevant for drainage culverts to serve as efficient road crossing structures for mammals?	Brunen B, Daguet C, Jaeger JAG	32510423 GEOGRAPHY
16	The Role of the Size of the Small Group and Informal Subgroups in Intragroup Conflicts.	Sidorenkov AV, Borokhovski EF	32353947 PSYCHOLOGY
17	Top-down and bottom-up controls on an herbivore on a native and introduced plant in a tropical agricultural landscape.	Despland E, Santacruz PG	32206453 BIOLOGY
18	Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids.	Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ	26981892 CSFG
19	A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.	Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ	30474973 CSFG
20	Performance monitoring in lung cancer patients pre- and post-chemotherapy using fine-grained electrophysiological measures	Simó M; Gurtubay-Antolin A; Vaquero L; Bruna J; Rodríguez-Fornells A;	29387526 MLNP
21	Tracking the microstructural properties of the main white matter pathways underlying speech processing in simultaneous interpreters	Elmer S; Hänggi J; Vaquero L; Cadena GO; François C; Rodríguez-Fornells A;	30831314 PSYCHOLOGY
22	Intra-operative Video Characterization of Carotid Artery Pulsation Patterns in Case Series with Post-endarterectomy Hypertension and Hyperperfusion Syndrome.	Xiao Y, Rivaz H, Kasuya H, Yokosako S, Mindru C, Teitelbaum J, Sirhan D, Sinclair D, Angle M, Lo BWY	29322480 PERFORM

Title:	A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.
Authors:	Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ
Link:	https://www.ncbi.nlm.nih.gov/pubmed/30474973?dopt=Abstract
DOI:	10.1021/acssynbio.8b00470
Publication:	ACS synthetic biology
Keywords:	cytochrome P450 monooxygenase; cytochrome P450 reductase; diterpenoid biosynthesis; steviol glucosides;
PMID:	30474973	Category:	ACS Synth Biol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Centre for Applied Synthetic Biology , Concordia University , Montréal , Québec H4B 1R6 , Canada. 2 Plant Biochemistry Laboratory, Department of Plant and Environmental Science , University of Copenhagen , DK-1871 Frederiksberg C , Denmark. 3 Center for Synthetic Biology , University of Copenhagen , DK-1871 Frederiksberg C , Denmark. 4 Centre for Structural and Functional Genomics , Concordia University , Montréal , Québec H4B 1R6 , Canada. 5 Evolva , Lersø Parkallé 42-44 , DK-2100 Copenhagen Ø , Denmark. 6 Department of Biology, Centre for Structural and Functional Genomic , Concordia University , Montréal , Québec H4B 1R6 , Canada.

Description:

A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929

Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Abstract

Biosynthesis of steviol glycosides in planta proceeds via two cytochrome P450 enzymes (CYPs): kaurene oxidase (KO) and kaurenoic acid hydroxylase (KAH). KO and KAH function in succession with the support of a NADPH-dependent cytochrome P450 reductase (CPR) to convert kaurene to steviol. This work describes a platform for recombinant production of steviol glucosides (SGs) in Saccharomyces cerevisiae, demonstrating the full reconstituted pathway from the simple sugar glucose to the SG precursor steviol. With a focus on optimization of the KO-KAH activities, combinations of functional homologues were tested in batch growth. Among the CYPs, novel KO75 (CYP701) and novel KAH82 (CYP72) outperformed their respective functional homologues from Stevia rebaudiana, SrKO (CYP701A5) and SrKAH (CYP81), in assays where substrate was supplemented to culture broth. With kaurene produced from glucose in the cell, SrCPR1 from S. rebaudiana supported highest turnover for KO-KAH combinations, besting two other CPRs isolated from S. rebaudiana, the Arabidopsis thaliana ATR2, and a new class I CPR12. Some coexpressions of ATR2 with a second CPR were found to diminish KAH activity, showing that coexpression of CPRs can lead to competition for CYPs with possibly adverse effects on catalysis.

PMID: 30474973 [PubMed - in process]

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