Keyword search (4,164 papers available)

"LC-MS" Keyword-tagged Publications:

Title Authors PubMed ID
1 International interlaboratory study to normalize liquid chromatography-based mycotoxin retention times through implementation of a retention index system Kelman MJ; Renaud JB; McCarron P; Hoogstra S; Chow W; Wang J; Varga E; Patriarca A; Vaya AM; Visintin L; Nguyen T; De Boevre M; De Saeger S; Karanghat V; Vuckovic D; McMullin DR; Dall' Asta C; Ayeni K; Warth B; Huang M; Tittlemier S; Mats L; Cao R; Sulyok M; Xu K; Berthiller F; Kuhn M; Cramer B; Ciasca B; Lattanzio V; De Baere S; Croubels S; DesRochers N; Sura S; Bates J; Wright EJ; Thapa I; Blackwell BA; Zhang K; Wong J; Burns L; Borts DJ; Sumarah MW; 39913989
CHEMBIOCHEM
2 Metabolomics 2023 workshop report: moving toward consensus on best QA/QC practices in LC-MS-based untargeted metabolomics Mosley JD; Dunn WB; Kuligowski J; Lewis MR; Monge ME; Ulmer Holland C; Vuckovic D; Zanetti KA; Schock TB; 38980450
CHEMBIOCHEM
3 Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics Mosley JD; Schock TB; Beecher CW; Dunn WB; Kuligowski J; Lewis MR; Theodoridis G; Ulmer Holland CZ; Vuckovic D; Wilson ID; Zanetti KA; 38345679
CHEMBIOCHEM
4 Metabolomics 2022 workshop report: state of QA/QC best practices in LC-MS-based untargeted metabolomics, informed through mQACC community engagement initiatives Dunn WB; Kuligowski J; Lewis M; Mosley JD; Schock T; Ulmer Holland C; Zanetti KA; Vuckovic D; 37940740
CHEMBIOCHEM
5 Comparative Analysis of Enzyme Production Patterns of Lignocellulose Degradation of Two White Rot Fungi: Obba rivulosa and Gelatoporia subvermispora Marinovíc M; Di Falco M; Aguilar Pontes MV; Gorzsás A; Tsang A; de Vries RP; Mäkelä MR; Hildén K; 35892327
CSFG
6 Dissemination and analysis of the quality assurance (QA) and quality control (QC) practices of LC-MS based untargeted metabolomics practitioners Evans AM; O' Donovan C; Playdon M; Beecher C; Beger RD; Bowden JA; Broadhurst D; Clish CB; Dasari S; Dunn WB; Griffin JL; Hartung T; Hsu PC; Huan T; Jans J; Jones CM; Kachman M; Kleensang A; Lewis MR; Monge ME; Mosley JD; Taylor E; Tayyari F; Theodoridis G; Torta F; Ubhi BK; Vuckovic D; 33044703
CONCORDIA
7 Proteomic Analysis of Morphologically Changed Tissues after Prolonged Dexamethasone Treatment Malkawi AK; Masood A; Shinwari Z; Jacob M; Benabdelkamel H; Matic G; Almuhanna F; Dasouki M; Alaiya AA; Rahman AMA; 31247941
CHEMBIOCHEM
8 Identification of novel enzymes to enhance the ruminal digestion of barley straw Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA; 29621684
CSFG
9 Mass Spectrometry-Based Proteomics Marcos Rafael Di Falco 29876812
CSFG

 

Title:Identification of novel enzymes to enhance the ruminal digestion of barley straw
Authors:Badhan ARibeiro GOJones DRWang YAbbott DWDi Falco MTsang AMcAllister TA
Link:https://pubmed.ncbi.nlm.nih.gov/29621684/
DOI:10.1016/j.biortech.2018.03.086
Publication:Bioresource technology
Keywords:Blue Native PAGECarbohydrate active enzymes (CAZymes)Fiber utilizationGlycosyl hydrolaseLC-MS/MSRumen nutrition
PMID:29621684 Category:Bioresour Technol Date Added:2019-06-07
Dept Affiliation: CSFG
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada.
3 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiome could enable formulation of enzyme cocktails that improve fiber digestion in ruminants. Compared to untreated barley straw, Viscozyme® increased gas production, dry matter digestion (P < 0.01) and volatile fatty acid production (P < 0.001) in ruminal batch cultures. Fractionation of Viscozyme® by Blue Native PAGE and analyses using a microassay and mass-spectrometry revealed a GH74 endoglucanase, GH71 a-1,3-glucanase, GH5 mannanase, GH7 cellobiohydrolase, GH28 pectinase, and esterases from Viscozyme® contributed to enhanced saccharification of barley straw by rumen mix enzymes. Grouping of these identified activities with their carbohydrate active enzymes (CAZy) counterparts enabled selection of similar CAZymes for downstream production and screening. Mining of these specific activities from other biological systems could lead to high value enzyme formulations for ruminants.





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