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PubMed Publications| Keyword search (4,164 papers available) |  |
"Tide" Keyword-tagged Publications:
| Title: | Hydrated electrons induce the formation of interstrand cross-links in DNA modified by cisplatin adducts | ||||
| Authors: | Behmand B, Noronha AM, Wilds CJ, Marignier JL, Mostafavi M, Wagner JR, Hunting DJ, Sanche L | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/32211848/ | ||||
| DOI: | 10.1093/jrr/rraa014 | ||||
| Publication: | Journal of radiation research | ||||
| Keywords: | anticancer drugs; gel electrophoresis; oligonucleotide; pulse radiolysis; radiotherapy; rate constant; | ||||
| PMID: | 32211848 | Category: | J Radiat Res | Date Added: | 2020-03-27 |
| Dept Affiliation: |
CHEMBIOCHEM
1 Groupe en sciences des radiations, Faculté de médicine et des sciences de la santé, Université de Sherbrooke, Sherbrooke, Québec, J1H 5N4, Canada. 2 Department of Chemistry and Biochemistry, Concordia University, Montréal, Québec, H4B1R6, Canada. 3 Centre de cinétique rapide ELYSE, Laboratoire de chimie physique, Université de Paris-Saclay 11, Orsay, France. |
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Description: |
Double-stranded oligonucleotides containing cisplatin adducts, with and without a mismatched region, were exposed to hydrated electrons generated by gamma-rays. Gel electrophoresis analysis demonstrates the formation of cisplatin-interstrand crosslinks from the cisplatin-intrastrand species. The rate constant per base for the reaction between hydrated electrons and the double-stranded oligonucleotides with and without cisplatin containing a mismatched region was determined by pulse radiolysis to be 7 × 109 and 2 × 109 M-1 s-1, respectively. These results provide a better understanding of the radiosensitizing effect of cisplatin adducts in hypoxic tumors and of the formation of interstrand crosslinks, which are difficult for cells to repair. |
