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PubMed Publications| Keyword search (4,163 papers available) |  |
"beta" Keyword-tagged Publications:
| Title: | The β2-adrenergic receptor agonist terbutaline upregulates T helper-17 cells in a protein kinase A-dependent manner | ||||
| Authors: | Carvajal Gonczi CM, Hajiaghayi M, Gholizadeh F, Xavier Soares MA, Touma F, Lopez Naranjo C, Rios AJ, Pozzebon C, Daigneault T, Burchell-Reyes K, Darlington PJ | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/37438188/ | ||||
| DOI: | 10.1016/j.humimm.2023.06.007 | ||||
| Publication: | Human immunology | ||||
| Keywords: | Beta-2-adrenergic receptor; CD4; IL-17A; Memory T cells; Terbutaline; | ||||
| PMID: | 37438188 | Category: | Date Added: | 2023-07-13 | |
| Dept Affiliation: |
PERFORM
1 Department of Biology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada. 2 Department of Psychology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada. 3 Department of Chemistry and Biochemistry, PERFORM Centre, Concordia University, Montreal, Quebec, Canada. 4 Department of Biology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada; Department of Psychology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada; Department of Health, Kinesiology & Applied Physiology, PERFORM Centre, Montreal, Quebec, Canada. Electronic address: peter.darlington@concordia.ca. |
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Description: |
Background: T helper 17 (Th17) cells produce IL-17A cytokine and can exacerbate autoimmune diseases and asthma. The ß2 adrenergic receptor is a g protein-coupled receptor that induces cAMP second messenger pathways. We tested the hypothesis that terbutaline, a ß2-adrenergic receptor-specific agonist, promotes IL-17 secretion by memory Th17 cells in a cAMP and PKA-dependent manner. Methods: Venous peripheral blood mononuclear cells (PBMC) from healthy human participants were activated with anti-CD3 and anti-CD28 antibodies. Secreted IL-17A was measured by enzyme linked immunosorbent assay, intracellular IL-17A, and ROR? were measured using flow cytometry, and RORC by qPCR. Memory CD3+CD4+CD45RA-CD45RO+ T cells were obtained by immunomagnetic negative selection and activated with tri-antibody complex CD3/CD28/CD2. Secreted IL-17A, intracellular IL-17A, RORC were measured, and phosphorylated-serine133-CREB was measured by western blotting memory Th cells. Results: Terbutaline increased IL-17A (p < 0.001), IL-17A+ cells (p < 0.05), and RORC in activated PBMC and memory Th cells. The PKA inhibitors H89 (p < 0.001) and Rp-cAMP (p < 0.01) abrogated the effects of terbutaline on IL-17A secretion in PBMC and memory T cells. Rolipram increased IL-17A (p < 0.01) to a similar extent as terbutaline. P-Ser133-CREB was increased by terbutaline (p < 0.05) in memory T cells. Conclusion: Terbutaline augments memory Th17 cells in lymphocytes from healthy participants. This could exacerbate autoimmune diseases or asthma, in cases where Th17 cells are considered to be pro-inflammatory. |
