PERFORM Publications

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Title		Authors	PubMed ID
1	Endangered species laws and the inclusion of Indigenous knowledges and sciences in risk assessments	Grimm J; Soares BE; Zanjani LV; Ballard M; Chiblow S; Andrade RS; Duncan AT; Fraser DJ; Mandrak NE; Bernos TA;	41684052 BIOLOGY
2	Strengthening community-based fisheries monitoring programs with Indigenous perspectives	Dewan K; Mulrennan ME; Georgekish E;	41332192 CONCORDIA
3	Characterization of muscle oxygenation response in well-trained handcyclists	Furno Puglia V; Paquette M; Bergdahl A;	38856729 HKAP
4	Cranberry supplementation improves physiological markers of performance in trained runners	Parenteau F; Puglia VF; Roberts M; Comtois AS; Bergdahl A;	38297471 HKAP
5	Identification of a Conserved Transcriptional Activator-Repressor Module Controlling the Expression of Genes Involved in Tannic Acid Degradation and Gallic Acid Utilization in Aspergillus niger	Arentshorst M; Falco MD; Moisan MC; Reid ID; Spaapen TOM; van Dam J; Demirci E; Powlowski J; Punt PJ; Tsang A; Ram AFJ;	37744122 CSFG
6	Electric Field-Induced Nano-Assembly Formation: First Evidence of Silicon Superclusters with a Giant Permanent Dipole Moment	Jardali F; Tran J; Liège F; Florea I; Leulmi ME; Vach H;	37570492 CERMM
7	Characterization of a novel AA3_1 xylooligosaccharide dehydrogenase from Thermothelomyces myriococcoides CBS 398.93	Zhao H; Karppi J; Nguyen TTM; Bellemare A; Tsang A; Master E; Tenkanen M;	36476312 CSFG
8	Metabolism of anti-inflammatory OXE (oxoeicosanoid) receptor antagonists by nonhuman primates	Cossette C; Chourey S; Ye Q; Reddy CN; Wang R; Poulet S; Slobodchikova I; Vuckovic D; Rokach J; Powell WS;	35158054 PERFORM
9	Discovery and Expression of Thermostable LPMOs from Thermophilic Fungi for Producing Efficient Lignocellulolytic Enzyme Cocktails.	Agrawal D, Basotra N, Balan V, Tsang A, Chadha BS	31792786 CSFG
10	Four Aromatic Intradiol Ring Cleavage Dioxygenases from Aspergillus niger.	Semana P, Powlowski J	31540981 CHEMISTRY
11	Malbranchea cinnamomea: A thermophilic fungal source of catalytically efficient lignocellulolytic glycosyl hydrolases and metal dependent enzymes.	Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS	26476165 CSFG
12	A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.	Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ	30474973 CSFG
13	In vivo α-hydroxylation of a 2-alkylindole antagonist of the OXE receptor for the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid in monkeys.	Chourey S, Ye Q, Reddy CN, Cossette C, Gravel S, Zeller M, Slobodchikova I, Vuckovic D, Rokach J, Powell WS	28476332 PERFORM
14	Metabolism and pharmacokinetics of a potent N-acylindole antagonist of the OXE receptor for the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE) in rats and monkeys.	Reddy CN, Alhamza H, Chourey S, Ye Q, Gore V, Cossette C, Gravel S, Slobodchikova I, Vuckovic D, Rokach J, Powell WS	29339225 PERFORM
15	Higher levels of cardiovascular fitness are associated with better executive function and prefrontal oxygenation in younger and older women.	Dupuy O, Gauthier CJ, Fraser SA, Desjardins-Crèpeau L, Desjardins M, Mekary S, Lesage F, Hoge RD, Pouliot P, Bherer L	25741267 PERFORM

Title:	A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.
Authors:	Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ
Link:	https://www.ncbi.nlm.nih.gov/pubmed/30474973?dopt=Abstract
DOI:	10.1021/acssynbio.8b00470
Publication:	ACS synthetic biology
Keywords:	cytochrome P450 monooxygenase; cytochrome P450 reductase; diterpenoid biosynthesis; steviol glucosides;
PMID:	30474973	Category:	ACS Synth Biol	Date Added:	2019-06-07
Dept Affiliation:	CSFG 1 Centre for Applied Synthetic Biology , Concordia University , Montréal , Québec H4B 1R6 , Canada. 2 Plant Biochemistry Laboratory, Department of Plant and Environmental Science , University of Copenhagen , DK-1871 Frederiksberg C , Denmark. 3 Center for Synthetic Biology , University of Copenhagen , DK-1871 Frederiksberg C , Denmark. 4 Centre for Structural and Functional Genomics , Concordia University , Montréal , Québec H4B 1R6 , Canada. 5 Evolva , Lersø Parkallé 42-44 , DK-2100 Copenhagen Ø , Denmark. 6 Department of Biology, Centre for Structural and Functional Genomic , Concordia University , Montréal , Québec H4B 1R6 , Canada.

Description:

A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929

Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Abstract

Biosynthesis of steviol glycosides in planta proceeds via two cytochrome P450 enzymes (CYPs): kaurene oxidase (KO) and kaurenoic acid hydroxylase (KAH). KO and KAH function in succession with the support of a NADPH-dependent cytochrome P450 reductase (CPR) to convert kaurene to steviol. This work describes a platform for recombinant production of steviol glucosides (SGs) in Saccharomyces cerevisiae, demonstrating the full reconstituted pathway from the simple sugar glucose to the SG precursor steviol. With a focus on optimization of the KO-KAH activities, combinations of functional homologues were tested in batch growth. Among the CYPs, novel KO75 (CYP701) and novel KAH82 (CYP72) outperformed their respective functional homologues from Stevia rebaudiana, SrKO (CYP701A5) and SrKAH (CYP81), in assays where substrate was supplemented to culture broth. With kaurene produced from glucose in the cell, SrCPR1 from S. rebaudiana supported highest turnover for KO-KAH combinations, besting two other CPRs isolated from S. rebaudiana, the Arabidopsis thaliana ATR2, and a new class I CPR12. Some coexpressions of ATR2 with a second CPR were found to diminish KAH activity, showing that coexpression of CPRs can lead to competition for CYPs with possibly adverse effects on catalysis.

PMID: 30474973 [PubMed - in process]

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