Keyword search (4,163 papers available)

"lipid" Keyword-tagged Publications:

Title Authors PubMed ID
1 Physical activity may protect myelin via modulation of high-density lipoprotein Boa Sorte Silva NC; Balbim GM; Stein RG; Gu Y; Tam RC; Dao E; Alkeridy W; Lam K; Kramer AF; Liu-Ambrose T; 39989020
HKAP
2 Neuroinflammation and oxidative redox imbalance drive memory dysfunction in adolescent rats prenatally exposed to Datura Stramonium Bamisi O; Oluwalabani AO; Arogundade TT; Olajide OJ; 39303770
PSYCHOLOGY
3 TANGO2 deficiency disease is predominantly caused by a lipid imbalance Sacher M; DeLoriea J; Mehranfar M; Casey C; Naaz A; Gamberi C; 38836374
BIOLOGY
4 Removal of Nutrients from Water Using Biosurfactant Micellar-Enhanced Ultrafiltration Binte Rafiq Era S; Mulligan CN; 36838547
ENCS
5 Overview of Sigma-1R Subcellular Specific Biological Functions and Role in Neuroprotection Véronik Lachance 36768299
CSBN
6 Utilization of a biosurfactant foam/nanoparticle mixture for treatment of oil pollutants in soil Vu KA; Mulligan CN; 35834082
ENCS
7 Mechanisms of hypericin incorporation to explain the photooxidation outcomes in phospholipid biomembrane models Pereira LSA; Camacho SA; Almeida AM; Gonçalves RS; Caetano W; DeWolf C; Aoki PHB; 35167859
CNSR
8 Caloric restriction causes a distinct reorganization of the lipidome in quiescent and non-quiescent cells of budding yeast Mohammad K; Orfanos E; Titorenko VI; 34853658
BIOLOGY
9 A green initiative for oiled sand cleanup using chitosan/rhamnolipid complex dispersion with pH-stimulus response Chen Z; An C; Wang Y; Zhang B; Tian X; Lee K; 34687682
ENCS
10 Body Mass Index Z Score vs Weight-for-Length Z Score in Infancy and Cardiometabolic Outcomes at Age 8-10 Years Roberge JB; Harnois-Leblanc S; McNealis V; van Hulst A; Barnett TA; Kakinami L; Paradis G; Henderson M; 34302856
PERFORM
11 Editorial: Advances in Dietary Fat Absorption Nauli AM; Santosa S; Dixon JB; 34168573
HKAP
12 Phase Diagram for a Lysyl-Phosphatidylglycerol Analogue in Biomimetic Mixed Monolayers with Phosphatidylglycerol: Insights into the Tunable Properties of Bacterial Membranes. Wölk C, Youssef H, Guttenberg T, Marbach H, Vizcay-Barrena G, Shen C, Brezesinski G, Harvey RD 32065707
CHEMBIOCHEM
13 Effects of chronic exposure to selenomethionine on social learning outcomes in zebrafish (Danio rerio): serotonergic dysregulation and oxidative stress in the brain. Attaran A, Salahinejad A, Naderi M, Crane AL, Niyogi S, Chivers DP 31972490
BIOLOGY
14 In Vivo Solid-Phase Microextraction for Sampling of Oxylipins in Brain of Awake, Moving Rats Napylov A; Reyes-Garces N; Gomez-Rios G; Olkowicz M; Lendor S; Monnin C; Bojko B; Hamani C; Pawliszyn J; Vuckovic D; 31697450
CHEMBIOCHEM
15 Mechanisms by which PE21, an extract from the white willow Salix alba, delays chronological aging in budding yeast. Medkour Y, Mohammad K, Arlia-Ciommo A, Svistkova V, Dakik P, Mitrofanova D, Rodriguez MEL, Junio JAB, Taifour T, Escudero P, Goltsios FF, Soodbakhsh S, Maalaoui H, Simard É, Titorenko VI 31645900
BIOLOGY
16 Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy. Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL 30317518
BIOLOGY
17 The Sexual Dimorphism of Lipid Kinetics in Humans. Santosa S, Jensen MD 26191040
PERFORM

 

Title:Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.
Authors:Mattie S,  Kazmirchuk T,  Mui J,  Vali H,  Brett CL
Link:https://www.ncbi.nlm.nih.gov/pubmed/30317518?dopt=Abstract
DOI:10.1007/978-1-4939-8760-3_24
Publication:Methods in molecular biology (Clifton, N.J.)
Keywords:Cryogenic electron microscopy (cryoEM);  Hemifusion;  Lipid bilayer merger;  Lysosome;  Membrane fusion;  SNARE;  Tomography;  Transmission electron microscopy (TEM);  Vacuole; 
PMID:30317518 Category:Methods Mol Biol Date Added:2019-06-07
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, Montréal, QC, Canada.
2 Montreal Neurological Hospital and Institute, McGill University, Montréal, QC, Canada.
3 Facility for Electron Microscopy Research, Department of Anatomy and Cell Biology, McGill University, Montréal, QC, Canada.
4 Department of Biology, Concordia University, Montréal, QC, Canada. christopher.brett@concordia.ca.

Description:

Visualization of SNARE-Mediated Organelle Membrane Hemifusion by Electron Microscopy.

Methods Mol Biol. 2019;1860:361-377

Authors: Mattie S, Kazmirchuk T, Mui J, Vali H, Brett CL

Abstract

SNARE-mediated membrane fusion is required for membrane trafficking as well as organelle biogenesis and homeostasis. The membrane fusion reaction involves sequential formation of hemifusion intermediates, whereby lipid monolayers partially mix on route to complete bilayer merger. Studies of the Saccharomyces cerevisiae lysosomal vacuole have revealed many of the fundamental mechanisms that drive the membrane fusion process, as well as features unique to organelle fusion. However, until recently, it has not been amenable to electron microscopy methods that have been invaluable for studying hemifusion in other model systems. Herein, we describe a method to visualize hemifusion intermediates during homotypic vacuole membrane fusion in vitro by transmission electron microscopy (TEM), electron tomography, and cryogenic electron microscopy (cryoEM). This method facilitates acquisition of invaluable ultrastructural data needed to comprehensively understand how fusogenic lipids and proteins contribute to SNARE-mediated membrane fusion-by-hemifusion and the unique features of organelle versus small-vesicle fusion.

PMID: 30317518 [PubMed - indexed for MEDLINE]




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