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PubMed Publications| Keyword search (4,163 papers available) |  |
"viability" Keyword-tagged Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Evaluation and Utilization of Aged Bacteria in MICP Technology | Fukue M; Lechowicz Z; Mulligan CN; Takeuchi S; Takeuchi H; | 41900613 ENCS |
| 2 | Properties and Behavior of Sandy Soils by a New Interpretation of MICP | Fukue M; Lechowicz Z; Mulligan CN; Takeuchi S; Fujimori Y; Emori K; | 40004331 ENCS |
| 3 | Cone allometry and seed protection from fire are similar in serotinous and nonserotinous conifers | Greene DF; Kane JM; Pounden E; Michaletz ST; | 38375897 BIOLOGY |
| 4 | Inhibited and Retarded Behavior by Ca2+ and Ca2+/OD Loading Rate on Ureolytic Bacteria in MICP Process | Fukue M; Lechowicz Z; Fujimori Y; Emori K; Mulligan CN; | 37176240 ENCS |
| 5 | Cytotoxicity and Genotoxicity of Azobenzene-Based Polymeric Nanocarriers for Phototriggered Drug Release and Biomedical Applications | Londoño-Berrío M; Pérez-Buitrago S; Ortiz-Trujillo IC; Hoyos-Palacio LM; Orozco LY; López L; Zárate-Triviño DG; Capobianco JA; Mena-Giraldo P; | 35956634 CNSR |
| 6 | Incorporation of Optical Density into the Blending Design for a Biocement Solution | Fukue M; Lechowicz Z; Fujimori Y; Emori K; Mulligan CN; | 35269187 ENCS |
| 7 | Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST) | Roversi K; Ebrahimi Orimi H; Falchetti M; Lummertz da Rocha E; Talbot S; Boutopoulos C; | 34442487 ENCS |
| 8 | Disturbance-induced emigration: an overlooked mechanism that reduces metapopulation extinction risk | Mestre A; Barfield M; Peniston JH; Peres-Neto PR; Mesquita-Joanes F; Holt RD; | 34086976 BIOLOGY |
| Title: | Bioprinting of Adult Dorsal Root Ganglion (DRG) Neurons Using Laser-Induced Side Transfer (LIST) | ||||
| Authors: | Roversi K, Ebrahimi Orimi H, Falchetti M, Lummertz da Rocha E, Talbot S, Boutopoulos C | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/34442487/ | ||||
| DOI: | 10.3390/mi12080865 | ||||
| Publication: | Micromachines | ||||
| Keywords: | adult DRG neurons; calcium kinetics; laser-assisted bioprinting; laser-induced side transfer; sensory neurons; transcriptome; viability; | ||||
| PMID: | 34442487 | Category: | Date Added: | 2021-08-27 | |
| Dept Affiliation: |
ENCS
1 Département de Pharmacologie et Physiologie, Université de Montréal, Montréal, QC H3C 3J7, Canada. 2 Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC H1T 2M4, Canada. 3 Department of Mechanical, Industrial and Aerospace Engineering, Concordia University, Montréal, QC H3G 1M8, Canada. 4 Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil. 5 Département d'Ophtalmologie, Université de Montréal, Montréal, QC H3C 3J7, Canada. 6 Institut de Génie Biomédical, Université de Montréal, Montréal, QC H3C 3J7, Canada. |
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Description: |
Cell bioprinting technologies aim to fabricate tissuelike constructs by delivering biomaterials layer-by-layer. Bioprinted constructs can reduce the use of animals in drug development and hold promise for addressing the shortage of organs for transplants. Here, we sought to validate the feasibility of bioprinting primary adult sensory neurons using a newly developed laser-assisted cell bioprinting technology, known as Laser-Induced Side Transfer (LIST). We used dorsal root ganglion neurons (DRG; cell bodies of somatosensory neurons) to prepare our bioink. DRG-laden- droplets were printed on fibrin-coated coverslips and their viability, calcium kinetics, neuropeptides release, and neurite outgrowth were measured. The transcriptome of the neurons was sequenced. We found that LIST-printed neurons maintain high viability (Printed: 86%, Control: 87% on average) and their capacity to release neuropeptides (Printed CGRP: 130 pg/mL, Control CGRP: 146 pg/mL). In addition, LIST-printed neurons do not show differences in the expressed genes compared to control neurons. However, in printed neurons, we found compromised neurite outgrowth and lower sensitivity to the ligand of the TRPV1 channel, capsaicin. In conclusion, LIST-printed neurons maintain high viability and marginal functionality losses. Overall, this work paves the way for bioprinting functional 2D neuron assays. |
