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Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.

Author(s): Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA

Biomed Res Int. 2015;2015:562952 Authors: Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA

Article GUID: 26180803
Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

Author(s): Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

N Biotechnol. 2018 Nov 25;46:1-6 Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Article GUID: 29803771
New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.

Author(s): Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

J Anim Sci. 2018 Jul 20;: Authors: Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

Article GUID: 30053012
Title:Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.
Authors:Badhan A,  Wang YX,  Gruninger R,  Patton D,  Powlowski J,  Tsang A,  McAllister TA
Link:https://www.ncbi.nlm.nih.gov/pubmed/26180803?dopt=Abstract
DOI:10.1155/2015/562952
Category:Biomed Res Int
PMID:26180803
Dept Affiliation: CSFG
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, AB, Canada T1H 4P4.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada H4B 1R6.

Description:

Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.

Biomed Res Int. 2015;2015:562952

Authors: Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA

Abstract

Identification of recalcitrant factors that limit digestion of forages and the development of enzymatic approaches that improve hydrolysis could play a key role in improving the efficiency of meat and milk production in ruminants. Enzyme fingerprinting of barley silage fed to heifers and total tract indigestible fibre residue (TIFR) collected from feces was used to identify cell wall components resistant to total tract digestion. Enzyme fingerprinting results identified acetyl xylan esterases as key to the enhanced ruminal digestion. FTIR analysis also suggested cross-link cell wall polymers as principal components of indigested fiber residues in feces. Based on structural information from enzymatic fingerprinting and FTIR, enzyme pretreatment to enhance glucose yield from barley straw and alfalfa hay upon exposure to mixed rumen-enzymes was developed. Prehydrolysis effects of recombinant fungal fibrolytic hydrolases were analyzed using microassay in combination with statistical experimental design. Recombinant hemicellulases and auxiliary enzymes initiated degradation of plant structural polysaccharides upon application and improved the in vitro saccharification of alfalfa and barley straw by mixed rumen enzymes. The validation results showed that microassay in combination with statistical experimental design can be successfully used to predict effective enzyme pretreatments that can enhance plant cell wall digestion by mixed rumen enzymes.

PMID: 26180803 [PubMed - indexed for MEDLINE]