Keyword search (3,939 papers available)


Importin-binding mediates the intramolecular regulation of anillin during cytokinesis.

Author(s): Beaudet D, Pham N, Skaik N, Piekny A

Mol Biol Cell. 2020 Apr 02;:mbcE20010006 Authors: Beaudet D, Pham N, Skaik N, Piekny A

Article GUID: 32238082
Active Ran regulates anillin function during cytokinesis.

Author(s): Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531 Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Article GUID: 28931593
Title:Active Ran regulates anillin function during cytokinesis.
Authors:Beaudet D,  Akhshi T,  Phillipp J,  Law C,  Piekny A
Link:https://www.ncbi.nlm.nih.gov/pubmed/28931593?dopt=Abstract
DOI:10.1091/mbc.E17-04-0253
Category:Mol Biol Cell
PMID:28931593
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, Montreal, QC H4B 1R6, Canada.
2 Program in Cell Biology, the Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
3 Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.
4 Centre for Microscopy and Cellular Imaging, Concordia University, Montreal, QC H4B 1R6, Canada.
5 Program in Cell Biology, the Hospital for Sick Children, Toronto, ON M5G 0A4, Canada alisa.piekny@concordia.ca.

Description:

Active Ran regulates anillin function during cytokinesis.

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531

Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Abstract

Cytokinesis cleaves a cell into two daughters at the end of mitosis, and must be spatially coordinated with chromosome segregation to prevent aneuploidy. The dogma is that the mitotic spindle governs the assembly and constriction of an actomyosin ring. Here, we reveal a function for active Ran in spatially restricting the ring. Our model is that during anaphase, "free" importins, whose gradient inversely correlates with active Ran and chromatin position, function as a molecular ruler for the recruitment and localization of anillin, a contractile protein and a crucial regulator of cytokinesis. We found that decreasing Ran-GTP levels or tethering active Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes. Anillin contains a conserved nuclear localization signal (NLS) at its C-terminus that binds to importin-ß and is required for cortical polarity and cytokinesis. Mutating the NLS decreases anillin's cortical affinity, causing it to be more dominantly regulated by microtubules. Anillin contains a RhoA-GTP binding domain, which autoinhibits the NLS and the neighboring microtubule-binding domain, and RhoA-GTP binding may relieve this inhibition during mitosis. Retention of the C-terminal NLS in anillin homologues suggests that this is a conserved mechanism for controlling anillin function.

PMID: 28931593 [PubMed - indexed for MEDLINE]