Keyword search (3,448 papers available)


The Canadian Fungal Research Network: current challenges and future opportunities.

Author(s): Horianopoulos LC, Gluck-Thaler E, Benoit Gelber I, Cowen LE, Geddes-McAlister J, Landry CR, Schwartz IS, Scott JA, Sellam A, Sheppard DC, Sp...

Can J Microbiol. 2020 Jul 27;: Authors: Horianopoulos LC, Gluck-Thaler E, Benoit Gelber I, Cowen LE, Geddes-McAlister J, Landry CR, Schwartz IS, Scott JA, Sellam A, Sheppard DC, Spribille T, Subra...

Article GUID: 32717148
Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space.

Author(s): Veri AO, Miao Z, Shapiro RS, Tebbji F, O'Meara TR, Kim SH, Colazo J, Tan K, Vyas VK, Whiteway M, Robbins N, Wong KH, Cowen LE

PLoS Genet. 2018 03;14(3):e1007270 Authors: Veri AO, Miao Z, Shapiro RS, Tebbji F, O'Meara TR, Kim SH, Colazo J, Tan K, Vyas VK, Whiteway M, Robbins N, Wong KH, Cowen LE

Article GUID: 29590106
Functional divergence of a global regulatory complex governing fungal filamentation.

Author(s): Polvi EJ, Veri AO, Liu Z, Hossain S, Hyde S, Kim SH, Tebbji F, Sellam A, Todd RT, Xie JL, Lin ZY, Wong CJ, Shapiro RS, Whiteway M, Robbins N...

Functional divergence of a global regulatory complex governing fungal filamentation.

PLoS Genet. 2019 01;15(1):e1007901

Authors: Polvi EJ, Veri AO, Liu Z, Hossain S, Hyde S, Kim...

Article GUID: 30615616
Beauvericin Potentiates Azole Activity via Inhibition of Multidrug Efflux, Blocks Candida albicans Morphogenesis, and Is Effluxed via Yor1 and Circuitry Controlled by Zcf29.

Author(s): Shekhar-Guturja T, Tebung WA, Mount H, Liu N, Köhler JR, Whiteway M, Cowen LE

Antimicrob Agents Chemother. 2016 12;60(12):7468-7480 Authors: Shekhar-Guturja T, Tebung WA, Mount H, Liu N, Köhler JR, Whiteway M, Cowen LE

Article GUID: 27736764
Title:Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space.
Authors:Veri AO,  Miao Z,  Shapiro RS,  Tebbji F,  O'Meara TR,  Kim SH,  Colazo J,  Tan K,  Vyas VK,  Whiteway M,  Robbins N,  Wong KH,  Cowen LE
Link:https://www.ncbi.nlm.nih.gov/pubmed/29590106?dopt=Abstract
DOI:10.1371/journal.pgen.1007270
Category:PLoS Genet
PMID:29590106
Dept Affiliation: BIOLOGY
1 Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
2 Faculty of Health Sciences, University of Macau, Macau SAR, China.
3 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
4 Infectious Disease Research Centre, Université Laval, Quebec City, Quebec, Canada.
5 Vanderbilt University School of Medicine, Nashville, Tennessee, United States of America.
6 Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, United States of America.
7 Department of Biology, Concordia University, Montréal, Quebec, Canada.

Description:

Tuning Hsf1 levels drives distinct fungal morphogenetic programs with depletion impairing Hsp90 function and overexpression expanding the target space.

PLoS Genet. 2018 03;14(3):e1007270

Authors: Veri AO, Miao Z, Shapiro RS, Tebbji F, O'Meara TR, Kim SH, Colazo J, Tan K, Vyas VK, Whiteway M, Robbins N, Wong KH, Cowen LE

Abstract

The capacity to respond to temperature fluctuations is critical for microorganisms to survive within mammalian hosts, and temperature modulates virulence traits of diverse pathogens. One key temperature-dependent virulence trait of the fungal pathogen Candida albicans is its ability to transition from yeast to filamentous growth, which is induced by environmental cues at host physiological temperature. A key regulator of temperature-dependent morphogenesis is the molecular chaperone Hsp90, which has complex functional relationships with the transcription factor Hsf1. Although Hsf1 controls global transcriptional remodeling in response to heat shock, its impact on morphogenesis remains unknown. Here, we establish an intriguing paradigm whereby overexpression or depletion of C. albicans HSF1 induces morphogenesis in the absence of external cues. HSF1 depletion compromises Hsp90 function, thereby driving filamentation. HSF1 overexpression does not impact Hsp90 function, but rather induces a dose-dependent expansion of Hsf1 direct targets that drives overexpression of positive regulators of filamentation, including Brg1 and Ume6, thereby bypassing the requirement for elevated temperature during morphogenesis. This work provides new insight into Hsf1-mediated environmentally contingent transcriptional control, implicates Hsf1 in regulation of a key virulence trait, and highlights fascinating biology whereby either overexpression or depletion of a single cellular regulator induces a profound developmental transition.

PMID: 29590106 [PubMed - indexed for MEDLINE]