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Human Mesenchymal Stem Cells Impact Th17 and Th1 Responses Through a Prostaglandin E2 and Myeloid-Dependent Mechanism.

Author(s): Rozenberg A, Rezk A, Boivin MN, Darlington PJ, Nyirenda M, Li R, Jalili F, Winer R, Artsy EA, Uccelli A, Reese JS, Planchon SM, Cohen JA, Bar-Or A

Stem Cells Transl Med. 2016 Nov;5(11):1506-1514 Authors: Rozenberg A, Rezk A, Boivin MN, Darlington PJ, Nyirenda M, Li R, Jalili F, Winer R, Artsy EA, Uccelli A, Reese JS, Planchon SM, Cohen JA, Bar-Or A

Article GUID: 27400792
Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging.

Author(s): Levtova N, Healy LM, Gonczi CMC, Stopnicki B, Blain M, Kennedy TE, Moore CS, Antel JP, Darlington PJ

J Neuroimmunol. 2017 09 15;310:143-149 Authors: Levtova N, Healy LM, Gonczi CMC, Stopnicki B, Blain M, Kennedy TE, Moore CS, Antel JP, Darlington PJ

Article GUID: 28606377
Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining.

Author(s): Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ

Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining.

J Vis Exp. 2014 Dec 23;(94):

Authors: Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ

Abstract<...

Article GUID: 25548935
Reciprocal modulation of helper Th1 and Th17 cells by the β2-adrenergic receptor agonist drug terbutaline.

Author(s): Carvajal Gonczi CM, Tabatabaei Shafiei M, East A, Martire E, Maurice-Ventouris MHI, Darlington PJ

FEBS J. 2017 09;284(18):3018-3028 Authors: Carvajal Gonczi CM, Tabatabaei Shafiei M, East A, Martire E, Maurice-Ventouris MHI, Darlington PJ

Article GUID: 28710773
Natural Killer Cells Regulate Th17 Cells After Autologous Hematopoietic Stem Cell Transplantation for Relapsing Remitting Multiple Sclerosis.

Author(s): Darlington PJ, Stopnicki B, Touil T, Doucet JS, Fawaz L, Roberts ME, Boivin MN, Arbour N, Freedman MS, Atkins HL, Bar-Or A

Front Immunol. 2018;9:834 Authors: Darlington PJ, Stopnicki B, Touil T, Doucet JS, Fawaz L, Roberts ME, Boivin MN, Arbour N, Freedman MS, Atkins HL, Bar-Or A

Article GUID: 29867923
Helper CD4 T cells expressing granzyme B cause glial fibrillary acidic protein fragmentation in astrocytes in an MHCII-independent manner.

Author(s): Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ

Glia. 2019 04;67(4):582-593 Authors: Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ

Article GUID: 30444064
Title:Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining.
Authors:Tabatabaei Shafiei M,  Carvajal Gonczi CM,  Rahman MS,  East A,  François J,  Darlington PJ
Link:www.ncbi.nlm.nih.gov/pubmed/25548935?dopt=Abstract
Category:J Vis Exp
PMID:25548935
Dept Affiliation: PERFORM
1 Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University.
2 Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University.
3 Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University.
4 Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University; Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University; peter.darlington@concordia.ca.

Description:

Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining.



J Vis Exp. 2014 Dec 23;(94):



Authors: Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ



Abstract

Periodic acid Schiff (PAS) staining is an immunohistochemical technique used on muscle biopsies and as a diagnostic tool for blood samples. Polysaccharides such as glycogen, glycoproteins, and glycolipids stain bright magenta making it easy to enumerate positive and negative cells within the tissue. In muscle cells PAS staining is used to determine the glycogen content in different types of muscle cells, while in blood cell samples PAS staining has been explored as a diagnostic tool for a variety of conditions. Blood contains a proportion of white blood cells that belong to the immune system. The notion that cells of the immune system possess glycogen and use it as an energy source has not been widely explored. Here, we describe an adapted version of the PAS staining protocol that can be applied on peripheral blood mononuclear immune cells from human venous blood. Small cells with PAS-positive granules and larger cells with diffuse PAS staining were observed. Treatment of samples with amylase abrogates these patterns confirming the specificity of the stain. An alternate technique based on enzymatic digestion confirmed the presence and amount of glycogen in the samples. This protocol is useful for hematologists or immunologists studying polysaccharide content in blood-derived lymphocytes.



PMID: 25548935 [PubMed - indexed for MEDLINE]