Keyword search (3,448 papers available)


Penicillium subrubescens adapts its enzyme production to the composition of plant biomass.

Author(s): Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP

Bioresour Technol. 2020 May 05;311:123477 Authors: Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP

Article GUID: 32408196

Evidence for ligninolytic activity of the ascomycete fungus Podospora anserina.

Author(s): van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA

Biotechnol Biofuels. 2020;13:75 Authors: van Erven G, Kleijn AF, Patyshakuliyeva A, Di Falco M, Tsang A, de Vries RP, van Berkel WJH, Kabel MA

Article GUID: 32322305

Glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens.

Author(s): Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, Mäkelä MR, de Vries RP

Appl Environ Microbiol. 2019 Oct 04;: Authors: Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, Mäkelä MR, de Vries RP

Article GUID: 31585998

Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila.

Author(s): Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A

Fungal Genet Biol. 2014 Nov;72:10-20 Authors: Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A

Article GUID: 24881579

Malbranchea cinnamomea: A thermophilic fungal source of catalytically efficient lignocellulolytic glycosyl hydrolases and metal dependent enzymes.

Author(s): Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS

Bioresour Technol. 2016 Jan;200:55-63 Authors: Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS

Article GUID: 26476165

Evaluation of secretome of highly efficient lignocellulolytic Penicillium sp. Dal 5 isolated from rhizosphere of conifers.

Author(s): Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS

Bioresour Technol. 2016 Sep;216:958-67 Authors: Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS

Article GUID: 27341464

The molecular response of the white-rot fungus Dichomitus squalens to wood and non-woody biomass as examined by transcriptome and exoproteome analyses.

Author(s): Rytioja J, Hildén K, Di Falco M, Zhou M, Aguilar-Pontes MV, Sietiö OM, Tsang A, de Vries RP, Mäkelä MR

Environ Microbiol. 2017 03;19(3):1237-1250 Authors: Rytioja J, Hildén K, Di Falco M, Zhou M, Aguilar-Pontes MV, Sietiö OM, Tsang A, de Vries RP, Mäkelä MR

Article GUID: 28028889

The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger.

Author(s): Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ

FEBS Lett. 2017 05;591(10):1408-1418 Authors: Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ

Article GUID: 28417461

Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

Author(s): Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

N Biotechnol. 2018 Nov 25;46:1-6 Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Article GUID: 29803771

The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum.

Author(s): Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP

N Biotechnol. 2019 Jul 25;51:57-66 Authors: Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP

Article GUID: 30797054


Title:Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.
Authors:Badhan AHuang JWang YAbbott DWDi Falco MTsang AMcAllister T
Link:https://www.ncbi.nlm.nih.gov/pubmed/29803771?dopt=Abstract
DOI:10.1016/j.nbt.2018.05.003
Category:N Biotechnol
PMID:29803771
Dept Affiliation: GENOMICS
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada.
2 Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, 330096, China.
3 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, H4B 1R6, Canada.
4 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

N Biotechnol. 2018 Nov 25;46:1-6

Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Abstract

In the present study, we have characterized high molecular weight multi-enzyme complexes in two commercial enzymes produced by Trichoderma reesei (Spezyme CP) and Penicillium funiculosum (Accellerase XC). We successfully identified 146-1000?kDa complexes using Blue native polyacrylamide gel electrophoresis (BN-PAGE) to fractionate the protein profile in both preparations. Identified complexes dissociated into lower molecular weight constituents when loaded on SDS PAGE. Unfolding of the secondary structure of multi-enzyme complexes with trimethylamine (pH >10) suggested that they were not a result of unspecific protein aggregation. Cellulase (CMCase) profiles of extracts of BN-PAGE fractionated protein bands confirmed cellulase activity within the multi-enzyme complexes. A microassay was used to identify protein bands that promoted high levels of glucose release from barley straw. Those with high saccharification yield were subjected to LC-MS analysis to identify the principal enzymatic activities responsible. The results suggest that secretion of proteins by aerobic fungi leads to the formation of high molecular weight multi-enzyme complexes that display activity against carboxymethyl cellulose and barley straw.

PMID: 29803771 [PubMed - indexed for MEDLINE]