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Author Correction: Building a global alliance of biofoundries.

Author(s): Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, John...

Nat Commun. 2019 Jul 11;10(1):3132 Authors: Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR,...

Article GUID: 31296848
Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis.

Author(s): Gold ND, Martin VJ

J Bacteriol. 2007 Oct;189(19):6787-95 Authors: Gold ND, Martin VJ

Article GUID: 17644599
Expression of a library of fungal β-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain.

Author(s): Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Appl Microbiol Biotechnol. 2012 Aug;95(3):647-59 Authors: Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Article GUID: 22218767
Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics.

Author(s): Gold ND, Gowen CM, Lussier FX, Cautha SC, Mahadevan R, Martin VJ

Microb Cell Fact. 2015 May 28;14:73 Authors: Gold ND, Gowen CM, Lussier FX, Cautha SC, Mahadevan R, Martin VJ

Article GUID: 26016674
Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids.

Author(s): Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

ACS Synth Biol. 2016 05 20;5(5):405-14 Authors: Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

Article GUID: 26981892
Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids.

Author(s): Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Methods Enzymol. 2016;575:195-224 Authors: Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Article GUID: 27417930
A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

Author(s): Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929 Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Article GUID: 30474973
Building a global alliance of biofoundries.

Author(s): Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Jo...

Nat Commun. 2019 05 09;10(1):2040 Authors: Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR...

Article GUID: 31068573
Title:Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids.
Authors:Pyne ME,  Narcross L,  Fossati E,  Bourgeois L,  Burton E,  Gold ND,  Martin VJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/27417930?dopt=Abstract
DOI:10.1016/bs.mie.2016.02.011
Category:Methods Enzymol
PMID:27417930
Dept Affiliation: GENOMICS
1 Centre for Structural and Functional Genomics, Concordia University, Montréal, QC, Canada.
2 Centre for Structural and Functional Genomics, Concordia University, Montréal, QC, Canada. Electronic address: vincent.martin@concordia.ca.

Description:

Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids.

Methods Enzymol. 2016;575:195-224

Authors: Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Abstract

Benzylisoquinoline alkaloids (BIAs) constitute a diverse class of plant secondary metabolites that includes the opiate analgesics morphine and codeine. Collectively, BIAs exhibit a myriad of pharmacological activities, including antimicrobial, antitussive, antispasmodic, and anticancer properties. Despite 2500 known BIA products, only a small proportion are currently produced though traditional crop-based manufacturing, as complex stereochemistry renders chemical synthesis of BIAs largely unfeasible. The advent of synthetic biology and sophisticated microbial engineering coupled with recent advances in the elucidation of plant BIA metabolic networks has provided growing motivation for producing high-value BIAs in microbial hosts. Here, we provide a technical basis for reconstituting BIA biosynthetic pathways in the common yeast Saccharomyces cerevisiae. Methodologies outlined in this chapter include fundamental techniques for expressing and assaying BIA biosynthetic enzymes, bioprospecting large libraries of BIA enzyme variants, and reconstituting and optimizing complete BIA formation pathways in yeast. To expedite construction of superior BIA-producing yeast strains, we emphasize high-throughput techniques. Finally, we identify fundamental challenges impeding deployment of yeast-based BIA production platforms and briefly outline future prospects to overcome such barriers.

PMID: 27417930 [PubMed - indexed for MEDLINE]