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Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.

Author(s): Warkentin R, Kwan DH

This review provides information on available methods for engineering glycan-binding proteins (GBP). Glycans are involved in a variety of physiological functions and are found in all domains of life and viruses. Due to their wide range of functions, GBPs ha...

Article GUID: 33450899
Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

Author(s): Soroko M, Kwan DH

Biochemistry. 2020 Jun 01;: Authors: Soroko M, Kwan DH

Article GUID: 32441090
Structure-Guided Directed Evolution of Glycosidases: A Case Study in Engineering a Blood Group Antigen-Cleaving Enzyme.

Author(s): Kwan DH

Methods Enzymol. 2017;597:25-53 Authors: Kwan DH

Article GUID: 28935105
Title:Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.
Authors:Warkentin R,  Kwan DH
Link:https://www.ncbi.nlm.nih.gov/pubmed/33450899
DOI:10.3390/molecules26020380
Category:Molecules
PMID:33450899
Dept Affiliation: CHEMBIOCHEM
1 Department of Biology, Centre for Applied Synthetic Biology, and Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada.
2 PROTEO, Quebec Network for Research on Protein Function, Structure, and Engineering, Quebec City, QC G1V 0A6, Canada.
3 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada.

Description:

Resources and Methods for Engineering "Designer" Glycan-Binding Proteins.

Molecules. 2021 Jan 13; 26(2):

Authors: Warkentin R, Kwan DH

Abstract

This review provides information on available methods for engineering glycan-binding proteins (GBP). Glycans are involved in a variety of physiological functions and are found in all domains of life and viruses. Due to their wide range of functions, GBPs have been developed with diagnostic, therapeutic, and biotechnological applications. The development of GBPs has traditionally been hindered by a lack of available glycan targets and sensitive and selective protein scaffolds; however, recent advances in glycobiology have largely overcome these challenges. Here we provide information on how to approach the design of novel "designer" GBPs, starting from the protein scaffold to the mutagenesis methods, selection, and characterization of the GBPs.

PMID: 33450899 [PubMed - as supplied by publisher]