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Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.

Author(s): Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemin KA, Tsang A, Yang W, McAllister TA

Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.
J Anim Sci. 2019 Jun 28;:
Authors: Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemi...

Article GUID: 31251799
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Author(s): Pereira EO, Tsang A, McAllister TA, Menassa R

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Article GUID: 23915965
Improvement in Saccharification Yield of Mixed Rumen Enzymes by Identification of Recalcitrant Cell Wall Constituents Using Enzyme Fingerprinting.

Author(s): Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA

Biomed Res Int. 2015;2015:562952 Authors: Badhan A, Wang YX, Gruninger R, Patton D, Powlowski J, Tsang A, McAllister TA

Article GUID: 26180803
Identification of Genes Involved in the Degradation of Lignocellulose Using Comparative Transcriptomics.

Author(s): Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA

Methods Mol Biol. 2017;1588:279-298 Authors: Gruninger RJ, Reid I, Forster RJ, Tsang A, McAllister TA

Article GUID: 28417376
Discovery and characterization of family 39 glycoside hydrolases from rumen anaerobic fungi with polyspecific activity on rare arabinosyl substrates.

Author(s): Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW

J Biol Chem. 2017 07 28;292(30):12606-12620 Authors: Jones DR, Uddin MS, Gruninger RJ, Pham TTM, Thomas D, Boraston AB, Briggs J, Pluvinage B, McAllister TA, Forster RJ, Tsang A, Selinger LB, Abbott DW

Article GUID: 28588026
New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.

Author(s): Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

J Anim Sci. 2018 Jul 20;: Authors: Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

Article GUID: 30053012
Title:Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.
Authors:Ran T,  Saleem AM,  Shen Y,  Ribeiro GO,  Beauchemin KA,  Tsang A,  Yang W,  McAllister TA
Link:www.ncbi.nlm.nih.gov/pubmed/31251799?dopt=Abstract
DOI:10.1093/jas/skz216
Category:J Anim Sci
PMID:31251799
Dept Affiliation: GENOMICS
1 Lethbridge Research and Development Centre, Lethbridge, AB, Canada.
2 Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, Hunan, China.
3 Department of Animal and Poultry Production, Faculty of Agriculture, South Valley University, Qena, Egypt.
4 College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
5 Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
6 Centre for Structural and Functional Genomic, Concordia University, Montreal, QC, Canada.

Description:

Effects of a recombinant fibrolytic enzyme on fiber digestion, ruminal fermentation, nitrogen balance and total tract digestibility of heifers fed a high forage diet.

J Anim Sci. 2019 Jun 28;:

Authors: Ran T, Saleem AM, Shen Y, Ribeiro GO, Beauchemin KA, Tsang A, Yang W, McAllister TA

Abstract

A metabolism study was conducted using 8 ruminal cannulated beef heifers to investigate the effects of a recombinant fibrolytic enzyme (RFE; xylanase XYL10C) selected specifically for forage fed ruminants on ruminal pH, fermentation, nitrogen balance and total tract digestibility of heifers. The experiment was a cross-over design with 2 treatments and 2 periods. The two treatments were a basal diet containing 60% barley silage, 30% barley straw and 10% supplement (DM basis) without (control) or with RFE. The enzyme was sprayed onto the barley straw at a rate of 6.6 × 104 IU/kg DM 24 h before feeding. Each period comprised 2 weeks of diet adaptation and 1 week of sampling and data collection. Feed intake and total tract digestibility of DM, OM, NDF and ADF were unaffected by RFE. Ruminal pH including mean, minimum, maximum, and duration pH < 5.8 did not differ between treatments. Total VFA concentration, molar proportion of individual VFA and acetate to propionate ratio were also not affected by RFE. However, ruminal NH3-N concentration (P < 0.06) and endoglucanase activity (P < 0.08) in ruminal fluid tended to be higher with RFE. Nitrogen utilization and microbial protein synthesis were not affected by treatment. These results indicate that XYL10C did not improve fiber digestion in heifers fed a high forage diet, despite the fact that it was specifically selected for this trait in laboratory assays. However, the increased ruminal NH3-N concentration suggests it potentially increased ruminal proteolytic activity.

PMID: 31251799 [PubMed - as supplied by publisher]