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Mycothermus thermophilus (Syn. Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed

Author(s): Neha Basotra

Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based product...

Article GUID: 27744242
Title:Mycothermus thermophilus (Syn. Scytalidium thermophilum): Repertoire of a diverse array of efficient cellulases and hemicellulases in the secretome revealed
Authors:Neha Basotra
Link:https://pubmed.ncbi.nlm.nih.gov/27744242/
DOI:10.1016/j.biortech.2016.10.018
Category:Bioresour Technol
PMID:27744242
Dept Affiliation: GENOMICS
1 Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: nehabasotra506@gmail.com.
2 Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: baljit_sohal@rediffmail.com.
3 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. Electronic address: marcos.difalco@concordia.ca.
4 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec H4B 1R6, Canada. Electronic address: adrian.tsang@concordia.ca.
5 Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, Punjab, India. Electronic address: chadhabs@yahoo.com.

Description:

Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based production medium. The sequential and differential expression profile of endoglucanases, ß-glucosidases, cellobiohydrolases and xylanases using zymography was studied. Mass spectrometry analysis of secretome (Q-TOF LC/MS) revealed a total of 240...