Keyword search (3,448 papers available)


Penicillium subrubescens adapts its enzyme production to the composition of plant biomass.

Author(s): Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP

Bioresour Technol. 2020 May 05;311:123477 Authors: Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP

Article GUID: 32408196
Evolutionary adaptation of Aspergillus niger for increased ferulic acid tolerance.

Author(s): Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP

J Appl Microbiol. 2019 Nov 01;: Authors: Lubbers RJM, Liwanag AJ, Peng M, Dilokpimol A, Benoit-Gelber I, de Vries RP

Article GUID: 31674709
Glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens.

Author(s): Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, Mäkelä MR, de Vries RP

Appl Environ Microbiol. 2019 Oct 04;: Authors: Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, Mäkelä MR, de Vries RP

Article GUID: 31585998
The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger.

Author(s): Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ

FEBS Lett. 2017 05;591(10):1408-1418 Authors: Alazi E, Khosravi C, Homan TG, du Pré S, Arentshorst M, Di Falco M, Pham TTM, Peng M, Aguilar-Pontes MV, Visser J, Tsang A, de Vries RP, Ram AFJ

Article GUID: 28417461
The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum.

Author(s): Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP

N Biotechnol. 2019 Jul 25;51:57-66 Authors: Coconi Linares N, Di Falco M, Benoit-Gelber I, Gruben BS, Peng M, Tsang A, Mäkelä MR, de Vries RP

Article GUID: 30797054
Title:Glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens.
Authors:Daly P,  Peng M,  Di Falco M,  Lipzen A,  Wang M,  Ng V,  Grigoriev IV,  Tsang A,  Mäkelä MR,  de Vries RP
Link:https://www.ncbi.nlm.nih.gov/pubmed/31585998?dopt=Abstract
DOI:10.1128/AEM.01828-19
Category:Appl Environ Microbiol
PMID:31585998
Dept Affiliation: GENOMICS
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands.
2 Center for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada.
3 U.S. Department of Energy Joint Genome Institute, Walnut Creek, California, USA.
4 Department of Microbiology, University of Helsinki, Helsinki, Finland.
5 Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular Physiology, Utrecht University, Utrecht, The Netherlands r.devries@wi.knaw.nl.

Description:

Glucose-mediated repression of plant biomass utilization in the white-rot fungus Dichomitus squalens.

Appl Environ Microbiol. 2019 Oct 04;:

Authors: Daly P, Peng M, Di Falco M, Lipzen A, Wang M, Ng V, Grigoriev IV, Tsang A, Mäkelä MR, de Vries RP

Abstract

The extent of carbon catabolite repression (CCR) at a global level is unknown in wood-rotting fungi, which are critical to the carbon cycle and are a source of biotechnological enzymes. CCR occurs in the presence of sufficient concentrations of easily metabolizable carbon sources (e.g. glucose), down-regulating the expression of genes encoding enzymes involved in the breakdown of complex carbon sources. We investigated this phenomenon in the white-rot fungus Dichomitus squalens using transcriptomics and exo-proteomics. In D. squalens cultures, approximately 7% of genes were repressed in the presence of glucose compared to Avicel or xylan alone. The glucose-repressed genes included the essential components for utilization of plant biomass - Carbohydrate Active enZyme (CAZy) and carbon catabolic genes. The majority of polysaccharide degrading CAZy genes were repressed and included activities towards all major carbohydrate polymers present in plant cell walls, while also repression of ligninolytic genes occurred. The transcriptome-level repression of the CAZy genes observed on the Avicel cultures was strongly supported by exo-proteomics. Protease encoding genes were generally not glucose-repressed indicating their likely dominant role in scavenging for nitrogen rather than carbon. The extent of CCR is surprising given that D. squalens rarely experiences high free sugar concentrations in its woody environment and indicates that biotechnological use of D. squalens for modification of plant biomass would benefit from de-repressed or constitutively CAZymes-expressing strains.Importance White-rot fungi are critical to the carbon cycle because they can mineralise all wood components using enzymes that also have biotechnological potential. The occurrence of carbon catabolite repression (CCR) in white-rot fungi is poorly understood. Previously, CCR in wood-rotting fungi has only been demonstrated for a small number of genes. We demonstrated widespread glucose-mediated CCR of plant biomass utilisation in the white-rot fungus D. squalens This indicates that the CCR mechanism has been largely retained even though wood-rotting fungi rarely experience commonly considered CCR conditions in their woody environment. The general lack of repression of genes encoding proteases along with the reduction in secreted CAZymes during CCR suggested that the retention of CCR may be connected with the need to conserve nitrogen use while growing on nitrogen-scarce wood. The widespread repression indicates that de-repressed strains could be beneficial for enzyme production.

PMID: 31585998 [PubMed - as supplied by publisher]