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Using intracellular plasmonics to characterize nanomorphology in human cells.

Author(s): Sohrabi Kashani A, Piekny A, Packirisamy M

Determining the characteristics and localization of nanoparticles inside cells is crucial for nanomedicine design for cancer therapy. Hyperspectral imaging is a fast, straightforward, reliable, and accurate method to study the interactions of nanoparticles ...

Article GUID: 33365137
Multi-tissue patterning drives anterior morphogenesis of the C. elegans embryo.

Author(s): Grimbert S, Mastronardi K, Richard V, Christensen R, Law C, Zardoui K, Fay D, Piekny A

Complex structures derived from multiple tissue types are challenging to study in vivo, and our knowledge of how cells from different tissues are coordinated is limited. Model organisms have proven invaluable for improving our understanding of how chemical ...

Article GUID: 33309948
Anillin Controls the Rho Zone.

Author(s): Piekny A

Bioessays. 2020 Sep 06; :e2000193 Authors: Piekny A PMID: 32893380 [PubMed - as supplied by publisher]

Article GUID: 32893380
Importin-binding mediates the intramolecular regulation of anillin during cytokinesis.

Author(s): Beaudet D, Pham N, Skaik N, Piekny A

Mol Biol Cell. 2020 Apr 02;:mbcE20010006 Authors: Beaudet D, Pham N, Skaik N, Piekny A

Article GUID: 32238082
Complementary functions for the Ran gradient during division.

Author(s): Ozugergin I, Piekny A

Small GTPases. 2020 Feb 03;: Authors: Ozugergin I, Piekny A

Article GUID: 32013678
Active Ran regulates anillin function during cytokinesis.

Author(s): Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531 Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Article GUID: 28931593
Dual disassembly and biological evaluation of enzyme/oxidation-responsive polyester-based nanoparticulates for tumor-targeting delivery.

Author(s): Hong SH, Larocque K, Jaunky DB, Piekny A, Oh JK

Colloids Surf B Biointerfaces. 2018 Dec 01;172:608-617 Authors: Hong SH, Larocque K, Jaunky DB, Piekny A, Oh JK

Article GUID: 30223243
Title:Using intracellular plasmonics to characterize nanomorphology in human cells.
Authors:Sohrabi Kashani A,  Piekny A,  Packirisamy M
Link:https://www.ncbi.nlm.nih.gov/pubmed/33365137
DOI:10.1038/s41378-020-00219-w
Category:Microsyst Nanoeng
PMID:33365137
Dept Affiliation: BIOLOGY
1 Optical Bio-Microsystem Lab, Micro-Nano-Bio-Integration Center, Department of Mechanical, Industrial and Aerospace Engineering, Concordia University, 1455 De Maisonneuve Blvd. W., Montreal, QC H3G 1M8 Canada.
2 Department of Biology, Concordia University, 7141 Sherbrooke Street W., Montreal, QC H4B 1R6 Canada.

Description:

Using intracellular plasmonics to characterize nanomorphology in human cells.

Microsyst Nanoeng. 2020; 6:110

Authors: Sohrabi Kashani A, Piekny A, Packirisamy M

Abstract

Determining the characteristics and localization of nanoparticles inside cells is crucial for nanomedicine design for cancer therapy. Hyperspectral imaging is a fast, straightforward, reliable, and accurate method to study the interactions of nanoparticles and intracellular components. With a hyperspectral image, we could collect spectral information consisting of thousands of pixels in a short time. Using hyperspectral images, in this work, we developed a label-free technique to detect nanoparticles in different regions of the cell. This technique is based on plasmonic shifts taking place during the interaction of nanoparticles with the surrounding medium. The unique optical properties of gold nanoparticles, localized surface plasmon resonance bands, are influenced by their microenvironment. The LSPR properties of nanoparticles, hence, could provide information on regions in which nanoparticles are distributed. To examine the potential of this technique for intracellular detection, we used three different types of gold nanoparticles: nanospheres, nanostars and Swarna Bhasma (SB), an Indian Ayurvedic/Sidha medicine, in A549 (human non-small cell lung cancer) and HepG2 (human hepatocellular carcinoma) cells. All three types of particles exhibited broader and longer bands once they were inside cells; however, their plasmonic shifts could change depending on the size and morphology of particles. This technique, along with dark-field images, revealed the uniform distribution of nanospheres in cells and could provide more accurate information on their intracellular microenvironment compared to the other particles. The region-dependent optical responses of nanoparticles in cells highlight the potential application of this technique for subcellular diagnosis when particles with proper size and morphology are chosen to reflect the microenvironment effects properly.

PMID: 33365137 [PubMed]