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Schizosaccharomyces pombe contains separate CC- and A-adding tRNA nucleotidyltransferases.

Author(s): Reid NE, Ngou JS, Joyce PBM

Biochem Biophys Res Commun. 2019 Jan 15;508(3):785-790 Authors: Reid NE, Ngou JS, Joyce PBM

Article GUID: 30528393
Analysis of the pathogenic I326T variant of human tRNA nucleotidyltransferase reveals reduced catalytic activity and thermal stability in vitro linked to a conformational change.

Author(s): Leibovitch M, Reid NE, Victoria J, Hanic-Joyce PJ, Joyce PBM

Biochim Biophys Acta Proteins Proteom. 2019 Jun;1867(6):616-626 Authors: Leibovitch M, Reid NE, Victoria J, Hanic-Joyce PJ, Joyce PBM

Article GUID: 30959222
Title:Schizosaccharomyces pombe contains separate CC- and A-adding tRNA nucleotidyltransferases.
Authors:Reid NE,  Ngou JS,  Joyce PBM
Link:https://www.ncbi.nlm.nih.gov/pubmed/30528393?dopt=Abstract
Category:Biochem Biophys Res Commun
PMID:30528393
Dept Affiliation: CHEMBIOCHEM
1 Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke St. W, Montréal, Québec, H4B 1R6, Canada.
2 Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke St. W, Montréal, Québec, H4B 1R6, Canada. Electronic address: paul.joyce@concordia.ca.

Description:

Schizosaccharomyces pombe contains separate CC- and A-adding tRNA nucleotidyltransferases.

Biochem Biophys Res Commun. 2019 Jan 15;508(3):785-790

Authors: Reid NE, Ngou JS, Joyce PBM

Abstract

A specific cytidine-cytidine-adenosine (CCA) sequence is required at the 3'-terminus of all functional tRNAs. This sequence is added during tRNA maturation or repair by tRNA nucleotidyltransferase enzymes. While most eukaryotes have a single enzyme responsible for CCA addition, some bacteria have separate CC- and A-adding activities. The fungus, Schizosaccharomyces pombe, has two genes (cca1 and cca2) that are thought, based on predicted amino acid sequences, to encode tRNA nucleotidyltransferases. Here, we show that both genes together are required to complement a Saccharomyces cerevisiae strain bearing a null mutation in the single gene encoding its tRNA nucleotidyltransferase. Using enzyme assays we show further that the purified S. pombe cca1 gene product specifically adds two cytidine residues to a tRNA substrate lacking this sequence while the cca2 gene product specifically adds the terminal adenosine residue thereby completing the CCA sequence. These data indicate that S. pombe represents the first eukaryote known to have separate CC- and A-adding activities for tRNA maturation and repair. In addition, we propose that a novel structural change in a tRNA nucleotidyltransferase is responsible for defining a CC-adding enzyme.

PMID: 30528393 [PubMed - in process]