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Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry

Author(s): Robert J Gruninger

Fungi utilize a unique mechanism of nutrient acquisition involving extracellular digestion. To understand the biology of these microbes, it is important to identify and characterize the function of proteins that are secreted and involved in this process. Ma...

Article GUID: 28417377
Title:Isolation and Preparation of Extracellular Proteins from Lignocellulose Degrading Fungi for Comparative Proteomic Studies Using Mass Spectrometry
Authors:Robert J Gruninger
Link:https://pubmed.ncbi.nlm.nih.gov/28417377/
DOI:10.1007/978-1-4939-6899-2_22
Category:Methods Mol Biol
PMID:28417377
Dept Affiliation: GENOMICS
1 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Ave. South, Lethbridge, AB, Canada, T1J 4B1. robert.gruninger@agr.gc.ca.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.
3 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, 5403 1st Ave. South, Lethbridge, AB, Canada, T1J 4B1.

Description:

Fungi utilize a unique mechanism of nutrient acquisition involving extracellular digestion. To understand the biology of these microbes, it is important to identify and characterize the function of proteins that are secreted and involved in this process. Mass spectrometry-based proteomics is a powerful tool to study complex mixtures of proteins and understand how the proteins produced by an organism change in response to different conditions. Many fungi are efficient decomposers of plant cell...