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Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.

Author(s): Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Appl Microbiol Biotechnol. 2007 May;75(2):337-46 Authors: Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Article GUID: 17333176
Expression of a library of fungal β-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain.

Author(s): Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Appl Microbiol Biotechnol. 2012 Aug;95(3):647-59 Authors: Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Article GUID: 22218767
Development of a pyrG mutant of Aspergillus oryzae strain S1 as a host for the production of heterologous proteins.

Author(s): Ling SO, Storms R, Zheng Y, Rodzi MR, Mahadi NM, Illias RM, Abdul Murad AM, Abu Bakar FD

ScientificWorldJournal. 2013;2013:634317 Authors: Ling SO, Storms R, Zheng Y, Rodzi MR, Mahadi NM, Illias RM, Abdul Murad AM, Abu Bakar FD

Article GUID: 24381522
Enhancement of synthetic Trichoderma-based enzyme mixtures for biomass conversion with an alternative family 5 glycosyl hydrolase from Sporotrichum thermophile.

Author(s): Ye Z, Zheng Y, Li B, Borrusch MS, Storms R, Walton JD

PLoS One. 2014;9(10):e109885 Authors: Ye Z, Zheng Y, Li B, Borrusch MS, Storms R, Walton JD

Article GUID: 25295862
Title:Expression of a library of fungal β-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain.
Authors:Wilde C,  Gold ND,  Bawa N,  Tambor JH,  Mougharbel L,  Storms R,  Martin VJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/22218767?dopt=Abstract
DOI:10.1007/s00253-011-3788-z
Category:Appl Microbiol Biotechnol
PMID:22218767
Dept Affiliation: GENOMICS
1 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West, Montréal, Québec H4B 1R6, Canada.

Description:

Expression of a library of fungal ß-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain.

Appl Microbiol Biotechnol. 2012 Aug;95(3):647-59

Authors: Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Abstract

Converting cellulosic biomass to ethanol involves the enzymatic hydrolysis of cellulose and the fermentation of the resulting glucose. The yeast Saccharomyces cerevisiae is naturally ethanologenic, but lacks the enzymes necessary to degrade cellulose to glucose. Towards the goal of engineering S. cerevisiae for hydrolysis of and ethanol production from cellulose, 35 fungal ß-glucosidases (BGL) from the BGL1 and BGL5 families were screened for their ability to be functionally expressed and displayed on the cell surface. Activity assays revealed that the BGL families had different substrate specificities, with only the BGL1s displaying activity on their natural substrate, cellobiose. However, growth on cellobiose showed no correlation between the specific growth rates, the final cell titer, and the level of BGL1 activity that was expressed. One of the BGLs that expressed the highest levels of cellobiase activity, Aspergillus niger BGL1 (Anig-Bgl101), was then used for further studies directed at developing an efficient cellobiose-fermenting strain. Expressing Anig-Bgl101 from a plasmid yielded higher ethanol levels when secreted into the medium rather than anchored to the cell surface. In contrast, ethanol yields from anchored and secreted Anig-Bgl101 were comparable when integrated on the chromosome. Flow cytometry analysis revealed that chromosomal integration of Anig-Bgl101 resulted in a higher percentage of the cell population that displayed the enzyme but with overall lower expression levels.

PMID: 22218767 [PubMed - indexed for MEDLINE]